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In order to investigate this further, the expression of N\u2010methyl\u2010 <jats:sc>d<\/jats:sc>\u2010aspartate (NMDA) receptor subunits was studied during the first three post\u2010natal weeks when motoneurones are differentially vulnerable to injury following neonatal nerve crush compared to the adult. Unilateral nerve crush was carried out at day 2 after birth (P2) which causes a decrease of 66% in motoneurone number by 14\u2003days (P14). To study receptor expression in identified motoneurones, serial section analysis was carried out on retrogradely labelled common peroneal (CP) motoneurones by combined immunocytochemistry and <jats:italic>in situ<\/jats:italic> hybridization (ISH). mRNA levels were also quantified in homogenates from lumbar spinal cords in which the side ipsilateral to the crush was separated from the contralateral side. The NR1 subunit of the NMDA receptor was widely distributed in the spinal cord being expressed most strongly in motoneurone somata particularly during the neonatal period (P3\u2013P7). The NR2 subunits were also expressed at higher levels in the somata and dendrites of neonatal motoneurones compared to older animals. NR2B mRNA was expressed at low to moderate levels throughout the studied period whereas NR2A mRNA levels were low until P21. Following unilateral nerve crush, an initial decrease in NR1 mRNA occurred at one day after nerve crush (P3) in labelled CP motoneurones ipsilateral to the crush which was followed by a significant increase in NR1 subunit expression at 5\u2003days post\u2010injury. This increase was bilateral although reaching greater significance ipsilateral to the crush compared with sham\u2010operated animals. A significant increase in NR1 and NR2B mRNA post injury was also detected in spinal cord homogenates. In addition, the changes in levels of NR1 and NR2B mRNA were reflected by comparable bilateral changes at P7 in receptor protein determined by quantitative immunocytochemical analysis of NR1 and NR2 subunit expression in identified CP motoneurones indicating a co\u2010ordinated regulation of receptor subunits in response to injury.<\/jats:p>","DOI":"10.1046\/j.1365-2990.2000.00244.x","type":"journal-article","created":{"date-parts":[[2003,3,12]],"date-time":"2003-03-12T14:51:32Z","timestamp":1047480692000},"page":"258-272","source":"Crossref","is-referenced-by-count":21,"title":["Changes in expression of NMDA receptor subunits in the rat lumbar spinal cord following neonatal nerve injury"],"prefix":"10.1111","volume":"26","author":[{"given":"L.","family":"Virgo","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"Dekkers","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G. 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