{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,5]],"date-time":"2025-10-05T04:13:47Z","timestamp":1759637627761},"reference-count":19,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2001,12,25]],"date-time":"2001-12-25T00:00:00Z","timestamp":1009238400000},"content-version":"vor","delay-in-days":603,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasite Immunology"],"published-print":{"date-parts":[[2000,5]]},"abstract":"<jats:p> <jats:italic>Peanut and ConA lectins were used as ligands to isolate glycoproteins from detergent extracts of adult <\/jats:italic>Ostertagia ostertagi <jats:italic>membranes. As judged by their profiles following SDS\u2010PAGE, these fractions closely resembled the equivalents from <\/jats:italic>Haemonchus contortus<jats:italic> which are derived from the nematode intestinal cell microvillar membranes and which are highly protective when used as antigens. Groups of calves were immunized with the peanut and ConA binding fractions of <\/jats:italic>Ostertagia<jats:italic>, either as separate or pooled antigens mixed with QuilA as adjuvant. All calves, including controls immunized with adjuvant only, were challenged with a single dose of infective <\/jats:italic>Ostertagia <jats:italic>larvae and faecal egg counts were monitored for 5 weeks. In two experiments where the antigen fractions were pooled, moderate (30\u201350%), but statistically significant reductions in egg output were observed, but the number of worms was not diminished. No significant protection was observed in a third trial where groups of calves were immunized with peanut or ConA binding proteins given separately. Two further trials were conducted in sheep immunized with the same <\/jats:italic>Ostertagia <jats:italic>fractions but challenged with <\/jats:italic>Haemonchus<jats:italic>. Irrespective of whether they were administered separately or together, the <\/jats:italic>Ostertagia <jats:italic>antigens cross protected efficiently against <\/jats:italic>Haemonchus <jats:italic>reducing egg counts by between 81% and 97% and worm numbers by between 57% and 84%<\/jats:italic>.<\/jats:p>","DOI":"10.1046\/j.1365-3024.2000.00303.x","type":"journal-article","created":{"date-parts":[[2003,3,12]],"date-time":"2003-03-12T17:09:32Z","timestamp":1047488972000},"page":"239-247","source":"Crossref","is-referenced-by-count":38,"title":["Evaluation of immunization with gut membrane glycoproteins of <i>Ostertagia ostertagi<\/i> against homologous challenge in calves and against <i>Haemonchus contortus <\/i>in sheep"],"prefix":"10.1111","volume":"22","author":[{"family":"Smith","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"family":"Smith","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"family":"Pettit","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2001,12,25]]},"reference":[{"key":"e_1_2_6_2_2","unstructured":"ArmourJ.A.(1967)Ostertagia ostertagiinfections in the bovine: field and experimental studies. PhD Thesis University of Glasgow"},{"key":"e_1_2_6_3_2","unstructured":"ArmourJ.A.&OgbourneC.P.(1982) Bovine ostertagiasis: a review and annotated bibliography. Miscellaneous publication No 7 of the Commonwealth Institute of Parasitology. Commonwealth Agricultural Bureaux England"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0450.1969.tb00121.x"},{"key":"e_1_2_6_5_2","first-page":"1781","article-title":"Gastrointestinal nematode immunisation trials in cattle","volume":"40","author":"Herlich H.","year":"1979","journal-title":"American Journal of Veterinary Science"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/0020-7519(94)00196-U"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0166-6851(97)00074-1"},{"key":"e_1_2_6_8_2","unstructured":"McMichael\u2010PhilipsD. MunnE.A. GrahamM.(1995) Helminth parasite antigen with aminopeptidase\u2010like activity. International patent application. WO 95\/12671"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/0034-5288(94)90136-8"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0169-4758(99)01399-X"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0166-6851(96)02812-5"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0020-7519(98)00195-7"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/0034-5288(93)90025-B"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/0020-7519(93)90150-W"},{"key":"e_1_2_6_15_2","unstructured":"SmithW.D. SmithS.K. MurrayJ.M. LiddellS. KnoxD.P.(1993b) Vaccines. European Patent Application. No 93916110"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3024.1994.tb00345.x"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-3024.1999.00217.x"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-3024.2000.00277.x"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-4017(96)01147-8"},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/0304-4017(95)00909-4"}],"container-title":["Parasite Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1046%2Fj.1365-3024.2000.00303.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1046\/j.1365-3024.2000.00303.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,29]],"date-time":"2023-10-29T16:14:06Z","timestamp":1698596046000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1046\/j.1365-3024.2000.00303.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2000,5]]},"references-count":19,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2000,5]]}},"alternative-id":["10.1046\/j.1365-3024.2000.00303.x"],"URL":"https:\/\/doi.org\/10.1046\/j.1365-3024.2000.00303.x","archive":["Portico"],"relation":{},"ISSN":["0141-9838","1365-3024"],"issn-type":[{"value":"0141-9838","type":"print"},{"value":"1365-3024","type":"electronic"}],"subject":[],"published":{"date-parts":[[2000,5]]}}}