{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,18]],"date-time":"2026-05-18T11:29:02Z","timestamp":1779103742722,"version":"3.51.4"},"reference-count":20,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2001,12,25]],"date-time":"2001-12-25T00:00:00Z","timestamp":1009238400000},"content-version":"vor","delay-in-days":444,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasite Immunology"],"published-print":{"date-parts":[[2000,10,7]]},"abstract":"<jats:p> <jats:italic>This study investigated the effect of the excretory\u2013secretory product (ESP) of <\/jats:italic>Paragonimus westermani<jats:italic> newly excysted metacercariae (PwNEM) on IL\u20108 production of human mature eosinophils. Treatment of eosinophils with lower concentrations (0.3 and 1\u2003\u03bcg\/ml) of the ESP significantly (<\/jats:italic>P\u2003&lt;\u2003<jats:italic>0.01) induced IL\u20108 production, whereas treament of cells with higher concentrations (3 and 10\u2003\u03bcg\/ml) did not. This effect of the ESP was concentration\u2010dependent. Interestingly, the amount of IL\u20108 production released into the culture supernatants was inversely correlated with the rate of eosinophil survival. When eosinophils were cultured with the same concentrations of the ESP for 24\u2003h, the ESP resulted in eosinophil death in a dose\u2010dependent manner. To investigate whether high proteolytic activity of proteases in the ESP could cause little production of IL\u20108, 10\u2003\u03bcg\/ml of ESP was pretreated with heat at 100\u2003\u00b0C for 10\u2003min or 56\u2003\u00b0C for 30\u2003min to reduce its proteolytic activity. IL\u20108 production of eosinophils incubated with heat\u2010treated ESP was markedly increased comparable to that of cells treated with the lowest concentration used in this study. These findings suggest that the protease in the ESP of PwNEM pivotally regulates IL\u20108 production by controlling of eosinophil survival, depending on the amount of ESP released <\/jats:italic>in vivo<jats:italic>. Thus, the cysteine protease in the ESP of PwNEM could provide a novel role to control recruitment of inflammatory cells in larval\u2010infected lesions in human paragonimiasis<\/jats:italic>.<\/jats:p>","DOI":"10.1046\/j.1365-3024.2000.00337.x","type":"journal-article","created":{"date-parts":[[2003,3,12]],"date-time":"2003-03-12T17:08:52Z","timestamp":1047488932000},"page":"529-533","source":"Crossref","is-referenced-by-count":19,"title":["Proteolytic activity of cysteine protease in excretory\u2013secretory product of <i>Paragonimus westermani<\/i> newly excysted metacercariae pivotally regulates IL\u20108 production of human eosinophils"],"prefix":"10.1111","volume":"22","author":[{"given":"Myeong Heon","family":"Shin","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Soo 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