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In Balb\/c mice, flagellar pocket immunization resulted in partial resistance towards the challenge with a low dose of parasites. This was accompanied by the induction of specific IgG2a antibodies. In an attempt to discover protective parasite antigens, antiflagellar pocket serum was used for the screening of a <\/jats:italic>T. brucei<jats:italic> bloodstream form cDNA library constructed in the \u03bbgt11 bacteriophage expression system. Through antibody panning and VSG elimination, 15 specific cDNA inserts were selected. Most intriguing was the observation that in addition to two clones encoding the invariant surface glycoprotein 75 (ISG75), 10 out of 15 independently selected cDNA inserts encoded the trypanosome heat shock protein 60 (tHSP60)<\/jats:italic>.<\/jats:p>","DOI":"10.1046\/j.1365-3024.2000.00348.x","type":"journal-article","created":{"date-parts":[[2003,3,12]],"date-time":"2003-03-12T17:09:13Z","timestamp":1047488953000},"page":"639-650","source":"Crossref","is-referenced-by-count":29,"title":["Antibodies raised against the flagellar pocket fraction of <i>Trypanosoma brucei <\/i>preferentially recognize HSP60 in cDNA expression library"],"prefix":"10.1111","volume":"22","author":[{"given":"Magdalena","family":"Radwanska","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefan","family":"Magez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nathalie","family":"Dumont","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Annette","family":"Pays","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Derek","family":"Nolan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Etienne","family":"Pays","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2001,12,25]]},"reference":[{"key":"e_1_2_6_2_1","first-page":"177","article-title":"The pellicle and the membrane of the flagellum, flegellar adhesion zone, and flagellar pocket: functionally discrete surface domains of the bloodstream form of African trypanosomes","volume":"10","author":"Balber A.E.","year":"1990","journal-title":"Critical Reviews in Immunology"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/jlb.63.4.429"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(82)90046-4"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.micro.52.1.745"},{"key":"e_1_2_6_6_1","unstructured":"BretsherP.A.(1996) In:T\u2010cell subsets and cytokines interplay in infectious diseases ed. 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