{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,30]],"date-time":"2023-10-30T05:17:05Z","timestamp":1698643025155},"reference-count":25,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2001,12,25]],"date-time":"2001-12-25T00:00:00Z","timestamp":1009238400000},"content-version":"vor","delay-in-days":572,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Scand J Immunol"],"published-print":{"date-parts":[[2000,6]]},"abstract":"<jats:p>Using 50 samples of umbilical cord blood lymphocytes from Japanese donors, we analysed two human T\u2010cell receptor beta variable (<jats:italic>TCRBV<\/jats:italic>) genes, <jats:italic>BV6S4<\/jats:italic> and <jats:italic>BV6S5<\/jats:italic>, for their polymorphism, usage frequencies and CD4\/CD8 skewness. They showed contrasting CD4\/CD8 skewness, <jats:italic>BV6S4<\/jats:italic> to CD8<jats:sup>+<\/jats:sup> T cells and <jats:italic>BV6S5 <\/jats:italic>to CD4<jats:sup>+<\/jats:sup> T cells. Genotyping of the <jats:italic>BV6S4<\/jats:italic> alleles (<jats:italic>A1<\/jats:italic>, <jats:italic>A2<\/jats:italic> and <jats:italic>A3<\/jats:italic>) revealed two of the six possible <jats:italic>BV6S4<\/jats:italic> genotypes, <jats:italic>A1<\/jats:italic>\/<jats:italic>A2<\/jats:italic> and <jats:italic>A2<\/jats:italic>\/<jats:italic>A2<\/jats:italic>. Among the two <jats:italic>BV6S4<\/jats:italic> genotypes, no significant difference was detected in usage frequency or CD4\/CD8 skewness. On the other hand, genotyping of the <jats:italic>BV6S5<\/jats:italic> alleles (<jats:italic>A1<\/jats:italic> and <jats:italic>A2<\/jats:italic>) revealed all three possible <jats:italic>BV6S5<\/jats:italic> genotypes, <jats:italic>A1<\/jats:italic>\/<jats:italic>A1<\/jats:italic>, <jats:italic>A1<\/jats:italic>\/<jats:italic>A2<\/jats:italic> and <jats:italic>A2<\/jats:italic>\/<jats:italic>A2<\/jats:italic>, and the gene usage frequency was high, in the order <jats:italic>A1<\/jats:italic>\/<jats:italic>A1<\/jats:italic>\u2003&gt;\u2003<jats:italic>A1<\/jats:italic>\/<jats:italic>A2<\/jats:italic>\u2003&gt;\u2003<jats:italic>A2<\/jats:italic>\/<jats:italic>A2<\/jats:italic>. These results indicate that the amino acid substitutions in <jats:italic>BV6S5<\/jats:italic> (S36R and G70E) are in some way associated with the expression level of this gene. In the analysis of CD4\/CD8 skewness, the three <jats:italic>BV6S5<\/jats:italic> genotypes had similar skewness, indicating that <jats:italic>A1<\/jats:italic> alleles are expressed more frequently than <jats:italic>A2<\/jats:italic> alleles in both CD4<jats:sup>+<\/jats:sup> and CD8<jats:sup>+<\/jats:sup> T\u2010cell populations. Although <jats:italic>BV6S5<\/jats:italic> exhibits marked skewness to CD4<jats:sup>+<\/jats:sup> T cells, our results indicate that the higher expression of <jats:italic>A1<\/jats:italic> alleles is not associated with the increased ratio of CD4<jats:sup>+<\/jats:sup> T cells.<\/jats:p>","DOI":"10.1046\/j.1365-3083.2000.00717.x","type":"journal-article","created":{"date-parts":[[2003,3,12]],"date-time":"2003-03-12T20:14:23Z","timestamp":1047500063000},"page":"543-547","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Human T\u2010Cell Receptor <i>BV6<\/i> Gene Polymorphism in Relation to Expression Level and CD4\/CD8 Skewness"],"prefix":"10.1111","volume":"51","author":[{"family":"Obata","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"family":"Shiiba","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"family":"Iizuka","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"family":"Kashiwagi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"family":"Kurosu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"family":"Shimada","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"family":"Nishijima","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"family":"Tozawa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2001,12,25]]},"reference":[{"key":"e_1_2_6_2_2","first-page":"451","article-title":"Nomenclature Sub\u2010Committee on TCR Designation. 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