{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,10]],"date-time":"2026-03-10T11:02:40Z","timestamp":1773140560073,"version":"3.50.1"},"reference-count":0,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2001,12,25]],"date-time":"2001-12-25T00:00:00Z","timestamp":1009238400000},"content-version":"vor","delay-in-days":480,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Scand J Immunol"],"published-print":{"date-parts":[[2000,9]]},"abstract":"<jats:p>Few data are available regarding the induction of memory T\u2010lymphocyte responses in cattle following Bacille Calmette Gu\u00e9rin (BCG) vaccination. Studies of the immune response induced by BCG vaccination provide an insight into the basis of antimycobacterial immunity that could be exploited for the development of more effective vaccination strategies. We used autologous dendritic cells (DC) infected with <jats:italic>Mycobacterium bovis<\/jats:italic> Bacille Calmette Gu\u00e9rin (BCG) or pulsed with purified protein derivative from <jats:italic>M. bovis<\/jats:italic> (PPD\u2010B) or <jats:italic>M. avium<\/jats:italic> (PPD\u2010A) to assess responses of CD4<jats:sup>+<\/jats:sup>, CD8<jats:sup>+<\/jats:sup> and WC1<jats:sup>+<\/jats:sup>\u03b3\u03b4 TCR<jats:sup>+<\/jats:sup> lymphocytes from BCG vaccinated and nonvaccinated cattle. Mycobacteria\u2010specific CD4<jats:sup>+<\/jats:sup> and CD8<jats:sup>+<\/jats:sup>, but not WC1<jats:sup>+<\/jats:sup>\u03b3\u03b4 TCR<jats:sup>+<\/jats:sup>, memory T lymphocytes were demonstrated in BCG\u2010vaccinated cattle. CD4<jats:sup>+<\/jats:sup> and CD8<jats:sup>+<\/jats:sup> lymphocytes proliferated and produced interferon (IFN)\u2010\u03b3 in response to BCG\u2010infected or PPD\u2010B\u2010pulsed DC. Proliferative responses were greater for CD4<jats:sup>+<\/jats:sup> than CD8<jats:sup>+<\/jats:sup> lymphocytes, although secretion of IFN\u2010\u03b3 was higher from the CD8<jats:sup>+<\/jats:sup> T cells. Responses to PPD\u2010A\u2010pulsed DC were lower, with no CD8<jats:sup>+<\/jats:sup> response. Lymphocytes from nonvaccinated calves were also stimulated to proliferate by BCG\u2010infected DC, although the magnitude of proliferation was lower. The findings suggest that immunity to <jats:italic>M. bovis<\/jats:italic> induced by BCG vaccination in cattle may involve CD8<jats:sup>+<\/jats:sup> memory T cells which produce IFN\u2010\u03b3, as well as CD4<jats:sup>+<\/jats:sup> memory T cells.<\/jats:p>","DOI":"10.1046\/j.1365-3083.2000.00780.x","type":"journal-article","created":{"date-parts":[[2003,3,12]],"date-time":"2003-03-12T20:15:12Z","timestamp":1047500112000},"page":"285-291","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":50,"title":["Dendritic Cells Induce CD4<sup>+<\/sup> and CD8<sup>+<\/sup> T\u2010Cell Responses to <i>Mycobacterium bovis<\/i> and <i>M. avium<\/i> Antigens in Bacille Calmette Gu\u00e9rin Vaccinated and Nonvaccinated Cattle"],"prefix":"10.1111","volume":"52","author":[{"family":"Hope","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"family":"Kwong","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"family":"Sopp","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"family":"Collins","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"family":"Howard","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2001,12,25]]},"container-title":["Scandinavian Journal of Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1046%2Fj.1365-3083.2000.00780.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1046\/j.1365-3083.2000.00780.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,29]],"date-time":"2023-10-29T11:14:57Z","timestamp":1698578097000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1046\/j.1365-3083.2000.00780.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2000,9]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2000,9]]}},"alternative-id":["10.1046\/j.1365-3083.2000.00780.x"],"URL":"https:\/\/doi.org\/10.1046\/j.1365-3083.2000.00780.x","archive":["Portico"],"relation":{},"ISSN":["0300-9475","1365-3083"],"issn-type":[{"value":"0300-9475","type":"print"},{"value":"1365-3083","type":"electronic"}],"subject":[],"published":{"date-parts":[[2000,9]]},"assertion":[{"value":"2001-12-25","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}