{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,30]],"date-time":"2023-10-30T05:10:52Z","timestamp":1698642652806},"reference-count":25,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2008,10,8]],"date-time":"2008-10-08T00:00:00Z","timestamp":1223424000000},"content-version":"vor","delay-in-days":2892,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Scand J Immunol"],"published-print":{"date-parts":[[2000,11,7]]},"abstract":"<jats:p>Exposure of Wistar rats to the immunotoxic compounds hexachlorobenzene (HCB), bis(tri\u2010<jats:italic>n<\/jats:italic>\u2010butyltin)oxide, and benzo(a)pyrene was previously found to affect mRNA expression of interleukin (IL)\u20102, IL\u20102R \u03b1\u2010chain, and interferon (IFN)\u2010\u03b3, the prototypic Th1 cytokine. In contrast, the mRNA expression of IL\u20104, the prototypic Th2 cytokine, was unaffected. This latter finding suggested that the IL\u20104 mRNA expression may not be an unequivocal parameter for Th2 responses in the rat. In order to obtain such a parameter the present study was performed, consisting of two types of experiments. Expression and production of IL\u20104 as well as IL\u201010, a second Th2 cytokine, were measured. First, Lewis (Th1 prone) and Brown Norway (BN; Th2 prone) rats were exposed to HCB. Exposure was previously found to increase the serum immunoglobulin (Ig)E levels, an IL\u20104\u2010dependent response, in BN but not Lewis rats, and in Lewis rats to aggravate experimental allergic encephalomyelitis (EAE), severity being inversely related to IL\u201010 levels. Secondly, BN rats were infected with <jats:italic>Trichinella spiralis<\/jats:italic>, an infection previously found to induce IL\u20104 production. HCB exposure did not affect IL\u20104 mRNA expression in either strain, while IL\u20104 production was decreased in Lewis and unaffected in BN rats. In Lewis rats both the mRNA expression and the production of IL\u201010 were decreased. The <jats:italic>T. spiralis<\/jats:italic> infection induced IL\u20104 and IL\u201010 mRNA expression, as well as IL\u201010 production. In contrast, the IL\u20104 production was strongly reduced. Thus, both the IL\u201010 mRNA expression and production correlated with the EAE development and <jats:italic>T. spiralis<\/jats:italic> infection. In HCB exposed Lewis rats and <jats:italic>T. spiralis<\/jats:italic> infected BN rats the IL\u20104 mRNA expression correlated with IgE levels and <jats:italic>T. spiralis<\/jats:italic> infection, respectively, whereas the IL\u20104 production lacked correlation in all cases. Collectively, these results suggest that IL\u201010 is an unequivocal Th2 parameter in the rat, whereas IL\u20104 is not.<\/jats:p>","DOI":"10.1046\/j.1365-3083.2000.00804.x","type":"journal-article","created":{"date-parts":[[2003,3,12]],"date-time":"2003-03-12T20:14:57Z","timestamp":1047500097000},"page":"519-524","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Interleukin\u201010 is an Unequivocal Th2 Parameter in the Rat, whereas Interleukin\u20104 is Not *"],"prefix":"10.1111","volume":"52","author":[{"given":"R. J.","family":"Vandebriel","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C.","family":"Meredith","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. 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