{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,18]],"date-time":"2023-10-18T06:30:48Z","timestamp":1697610648239},"reference-count":16,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2001,12,23]],"date-time":"2001-12-23T00:00:00Z","timestamp":1009065600000},"content-version":"vor","delay-in-days":297,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Scand J Immunol"],"published-print":{"date-parts":[[2001,3]]},"abstract":"<jats:p>Idiotypes are molecular clues used to explore the specificity and diversity of immune response. In the present study, anti\u2010idiotype antibodies were used to neutralize the pathogenic effects induced by the injection of pemphigus immunoglobulin(Ig)G into BALB\/<jats:italic>c<\/jats:italic> mice. To achieve our goal, antidesmoglein 1 IgG was obtained from a patient with <jats:italic>pemphigus foliaceus<\/jats:italic> with high titer of antiepithelial antibodies. The IgG was isolated by ion exchange chromatography, then digested by pepsin. F(ab\u2032)<jats:sub>2<\/jats:sub> fragments were purified in Sephacryl S\u2010300 and injected in rabbits to produce anti\u2010idiotype antibodies. The rabbit sera reacted with the pemphigus F(ab\u2032)<jats:sub>2<\/jats:sub> fragments. Eleven <jats:italic>pemphigus foliaceus<\/jats:italic> sera were recognized by the anti\u2010idiotype serum at the light or heavy chains whereas bullous pemphigoid and normal IgG were negative. Neonatal BALB\/<jats:italic>c<\/jats:italic> mice injected with pemphigus IgG developed intraepidermal blisters, mimicking the clinical and immunopathological features of the pemphigus. In contrast, the animals treated with anti\u2010idiotype antibodies and pemphigus IgG did not develop blisters. Thus, anti\u2010idiotype antibodies neutralize <jats:italic>in vivo<\/jats:italic> the pathogenic effects of pemphigus IgG.<\/jats:p>","DOI":"10.1046\/j.1365-3083.2001.00863.x","type":"journal-article","created":{"date-parts":[[2003,3,12]],"date-time":"2003-03-12T20:16:00Z","timestamp":1047500160000},"page":"254-258","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Anti\u2010Idiotype Antibodies Neutralize <i>In vivo<\/i> the Blistering Effect of <i>Pemphigus foliaceus<\/i> IgG"],"prefix":"10.1111","volume":"53","author":[{"given":"E.","family":"Alvarado\u2010Flores","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E.","family":"Avalos\u2010D\u00edaz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L. A.","family":"D\u00edaz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R.","family":"Herrera\u2010Esparza","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2001,12,23]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.3181\/00379727-117-29622"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2133.1984.tb04735.x"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.122.2.409"},{"key":"e_1_2_6_5_2","first-page":"319","article-title":"Pemphigus: autoimmunity to epidermal cell adhesion molecules","volume":"11","author":"Amagai M","year":"1996","journal-title":"Adv Dermatol"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2133.1982.tb04542.x"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM198205203062001"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI3647"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1006\/clin.1997.4407"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1006\/jaut.1996.0029"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1006\/jtbi.1998.0818"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/0022-1759(79)90326-0"},{"key":"e_1_2_6_13_2","doi-asserted-by":"crossref","first-page":"575","DOI":"10.1016\/0022-2836(73)90198-8","article-title":"Maturation of the head of bacteriophage T4 I DNA packaging events","volume":"80","author":"Laemmli UK","year":"1973","journal-title":"J Mol Biol"},{"key":"e_1_2_6_14_2","doi-asserted-by":"crossref","first-page":"4350","DOI":"10.1073\/pnas.76.9.4350","article-title":"Electrophoretic transfer of proteins from polyacrylamide gels to nitrocelulose sheets: Procedure and some applications","volume":"76","author":"Towbin HT","year":"1977","journal-title":"Proc Natl Acad Sci USA"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1111\/1523-1747.ep12476347"},{"key":"e_1_2_6_16_2","first-page":"418","article-title":"Production of anti\u2010idiotype antibodies in pemphigus","volume":"109","author":"Richard C","year":"1997","journal-title":"J Invest Dermatol"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2249.1999.00930.x"}],"container-title":["Scandinavian Journal of Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1046%2Fj.1365-3083.2001.00863.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1046%2Fj.1365-3083.2001.00863.x","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1046\/j.1365-3083.2001.00863.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,17]],"date-time":"2023-10-17T16:05:45Z","timestamp":1697558745000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1046\/j.1365-3083.2001.00863.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2001,3]]},"references-count":16,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2001,3]]}},"alternative-id":["10.1046\/j.1365-3083.2001.00863.x"],"URL":"https:\/\/doi.org\/10.1046\/j.1365-3083.2001.00863.x","archive":["Portico"],"relation":{},"ISSN":["0300-9475","1365-3083"],"issn-type":[{"value":"0300-9475","type":"print"},{"value":"1365-3083","type":"electronic"}],"subject":[],"published":{"date-parts":[[2001,3]]},"assertion":[{"value":"2001-12-23","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}