{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T20:31:50Z","timestamp":1791664310458,"version":"4.3.4"},"reference-count":45,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2001,12,25]],"date-time":"2001-12-25T00:00:00Z","timestamp":1009238400000},"content-version":"vor","delay-in-days":724,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Plant Journal"],"published-print":{"date-parts":[[2000,1]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>\n                    Disease resistance (\n                    <jats:italic>R<\/jats:italic>\n                    ) genes are found in plants as either simple (single allelic series) loci, or more frequently as complex loci of tandemly repeated genes. These different loci are likely to be under similar evolutionary forces from pathogens, but the contrast between them suggests important differences in mechanisms associated with DNA structure and recombination that generate and maintain\n                    <jats:italic>R<\/jats:italic>\n                    gene diversity. The\n                    <jats:italic>RPP13<\/jats:italic>\n                    locus in\n                    <jats:italic>Arabidopsis<\/jats:italic>\n                    represents an important paradigm for studying the evolution of an\n                    <jats:italic>R<\/jats:italic>\n                    gene at a simple locus. The\n                    <jats:italic>RPP13<\/jats:italic>\n                    allele from the accession Nd\u20101, designated\n                    <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"DDBJ\/EMBL\/GenBank\" xlink:href=\"RPP13-\">RPP13\u2010<\/jats:ext-link>\n                    <jats:italic>Nd<\/jats:italic>\n                    , confers resistance to five different isolates of the biotrophic oomycete,\n                    <jats:italic>Peronospora parasitica<\/jats:italic>\n                    (causal agent of downy mildew), and encodes an NBS\u2010LRR type R protein with a putative amino\u2010terminal leucine zipper. The\n                    <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"DDBJ\/EMBL\/GenBank\" xlink:href=\"RPP13-\">RPP13\u2010<\/jats:ext-link>\n                    <jats:italic>Rld<\/jats:italic>\n                    allele, cloned from the accession Rld\u20102, encodes a different specificity. Comparison of three\n                    <jats:italic>RPP13<\/jats:italic>\n                    alleles revealed a high rate of amino acid divergence within the LRR domain, less than 80% identity overall, compared to the remainder of the protein (&gt;95% identity). We also found evidence for positive selection in the LRR domain for amino acid diversification outside the core conserved \u03b2\u2010strand\/\u03b2\u2010turn motif, suggesting that more of the LRR structure is available for interaction with target molecules than has previously been reported for other\n                    <jats:italic>R<\/jats:italic>\n                    gene products. Furthermore, an amino acid sequence (LLRVLDL) identical in an LRR among\n                    <jats:italic>RPP13<\/jats:italic>\n                    alleles is conserved in other LZ NBS\u2010LRR type R proteins, suggesting functional significance.\n                  <\/jats:p>","DOI":"10.1046\/j.1365-313x.2000.00664.x","type":"journal-article","created":{"date-parts":[[2003,3,12]],"date-time":"2003-03-12T16:59:30Z","timestamp":1047488370000},"page":"177-188","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":268,"title":["RPP13\n                    <i>is a simple locus in<\/i>\n                    Arabidopsis thaliana\n                    <i>for alleles that specify downy mildew resistance to different avirulence determinants in<\/i>\n                    Peronospora parasitica"],"prefix":"10.1111","volume":"21","author":[{"given":"Peter D.","family":"Bittner\u2010Eddy","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ian R.","family":"Crute","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric B.","family":"Holub","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jim L.","family":"Beynon","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2001,12,25]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0959-437X(05)80275-8"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/25.17.3389"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.9.4.641"},{"key":"e_1_2_6_5_1","volume-title":"Struhl, K","author":"Ausubel F.M.","year":"1994"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.8091210"},{"key":"e_1_2_6_7_1","first-page":"1194","article-title":"In planta Agrobacterium\u2010mediated gene transfer by infiltration of Arabidopsis thaliana plants","volume":"316","author":"Betchtold N.","year":"1993","journal-title":"C. 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