{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,23]],"date-time":"2025-09-23T13:43:49Z","timestamp":1758635029624},"reference-count":25,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2001,12,25]],"date-time":"2001-12-25T00:00:00Z","timestamp":1009238400000},"content-version":"vor","delay-in-days":664,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transfusion Medicine"],"published-print":{"date-parts":[[2000,3]]},"abstract":"<jats:p>Donor leucocytes are responsible for many adverse transfusion effects. Clinical reactions may be attributed to specific leucocyte subsets. In this study leucocyte subpopulations were identified and quantified pre\u2010 and post\u2010leucodepletion by integral filtration using novel Optipac<jats:sup>\u00ae<\/jats:sup> configurations incorporating either WBF\u20101 (Pall Medsep) or RS2000 (Asahi) whole blood filters. Leucocytes were analysed by flow cytometry using direct, four\u2010colour, membrane immunofluorescence with monoclonal antibodies specific for CD 3, 14, 16, 19 and 45. \u2003Filtration reduced the leucocyte load by 3\u20134 log<jats:sub>10<\/jats:sub>, consistently giving products with &lt;\u20032 cells \u03bcL<jats:sup>\u22121<\/jats:sup>. Subset distributions were also affected with the proportion of neutrophils and monocytes increased and the lymphocyte\/monocyte ratio inverted. These effects were independent of the preprocessing hold conditions, filter used and buffy coat (BC) removal. All filtered red cell products contained 75\u201380% neutrophils, 16\u201320% monocytes and 2\u20137% lymphocytes. Results presented here demonstrate that whole blood filtration, and BC removal, significantly reduce the content and substantially alter the subpopulation distribution of the donor leucocytes remaining in leucodepleted red cell products.<\/jats:p>","DOI":"10.1046\/j.1365-3148.2000.00231.x","type":"journal-article","created":{"date-parts":[[2003,3,12]],"date-time":"2003-03-12T21:27:54Z","timestamp":1047504474000},"page":"49-58","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Differential leucocyte subpopulation analysis of leucodepleted red cell products"],"prefix":"10.1111","volume":"10","author":[{"family":"Rider","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"family":"Want","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"family":"Winter","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"family":"Turton","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"family":"Pamphilon","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"family":"Nobes","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2001,12,25]]},"reference":[{"key":"e_1_2_5_2_2","unstructured":"American Association of Blood Banks. (1996) Standards for Blood Banks and Transfusion Services 17th edn. AABB Bethesda."},{"key":"e_1_2_5_3_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1423-0410.1992.tb01206.x"},{"key":"e_1_2_5_4_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V84.6.1703.1703"},{"key":"e_1_2_5_5_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-3148.1998.00129.x"},{"key":"e_1_2_5_6_2","doi-asserted-by":"crossref","first-page":"4373","DOI":"10.4049\/jimmunol.162.8.4373","article-title":"Cutting edge: sustained expansion of CD8+ T cells requires CD154 expression of Th cells in acute graft versus host disease","volume":"162","author":"Buhlmann J.E.","year":"1999","journal-title":"Journal of Immunology"},{"key":"e_1_2_5_7_2","unstructured":"Department of Health. (1998) Government Accepts Advice on Leucodepletion from Spongiform Encephalopathy Advisory Committee. Press Release 98 \/295."},{"key":"e_1_2_5_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0887-7963(93)70125-X"},{"key":"e_1_2_5_9_2","unstructured":"Edinburgh Consensus Conference. (1993) Leucocyte depletion of blood and blood products. Royal College of Physicians of Edinburgh 18\u201319 March 1993."},{"key":"e_1_2_5_10_2","doi-asserted-by":"crossref","first-page":"2403","DOI":"10.1182\/blood.V86.6.2403.bloodjournal8662403","article-title":"Neutrophil Fc\u03b3RIIIb deficiency, nature, and clinical consequences: a study of 21 individuals from 14 families","volume":"86","author":"De Haas M.","year":"1995","journal-title":"Blood"},{"key":"e_1_2_5_11_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.1994.tb04923.x"},{"key":"e_1_2_5_12_2","doi-asserted-by":"crossref","first-page":"603","DOI":"10.1182\/blood.V85.3.603.bloodjournal853603","article-title":"The leukodepletion of cellular blood products in the prevention of HLA\u2010alloimmunization and refractoriness to allogenic platelet transfusions","volume":"85","author":"Heddle N.H.","year":"1995","journal-title":"Blood"},{"key":"e_1_2_5_13_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1537-2995.1991.31791368338.x"},{"issue":"1","key":"e_1_2_5_14_2","article-title":"Quantitiation of WBC subsets in leukoreduced red blood cells (abstract O17)","volume":"8","author":"Johnson R.","year":"1998","journal-title":"Transfusion Medicine"},{"key":"e_1_2_5_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1246-7820(98)80112-2"},{"key":"e_1_2_5_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/0268-960X(95)90035-7"},{"key":"e_1_2_5_17_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1423-0410.1997.7310028.x"},{"key":"e_1_2_5_18_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1423-0410.1994.tb00272.x"},{"key":"e_1_2_5_19_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2141.1996.d01-1785.x"},{"key":"e_1_2_5_20_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1423-0410.1995.tb02594.x"},{"key":"e_1_2_5_21_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1537-2995.1997.371098016442.x"},{"key":"e_1_2_5_22_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1537-2995.1995.35996029155.x"},{"key":"e_1_2_5_23_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1537-2995.1998.38798346633.x"},{"key":"e_1_2_5_24_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1537-2995.1991.31191096184.x"},{"key":"e_1_2_5_25_2","unstructured":"UK BTS. (1998) Guidelines for the Blood Transfusion Service. United Kingdom Blood Transfusion Service \/ National Institute for Biological Standards 2nd edn. HMSO London."},{"key":"e_1_2_5_26_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-3148.1999.009001051.x"}],"container-title":["Transfusion Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1046%2Fj.1365-3148.2000.00231.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1046\/j.1365-3148.2000.00231.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,29]],"date-time":"2023-10-29T11:31:01Z","timestamp":1698579061000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1046\/j.1365-3148.2000.00231.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2000,3]]},"references-count":25,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2000,3]]}},"alternative-id":["10.1046\/j.1365-3148.2000.00231.x"],"URL":"https:\/\/doi.org\/10.1046\/j.1365-3148.2000.00231.x","archive":["Portico"],"relation":{},"ISSN":["0958-7578","1365-3148"],"issn-type":[{"value":"0958-7578","type":"print"},{"value":"1365-3148","type":"electronic"}],"subject":[],"published":{"date-parts":[[2000,3]]},"assertion":[{"value":"2001-12-25","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}