{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,17]],"date-time":"2026-02-17T03:12:54Z","timestamp":1771297974689,"version":"3.50.1"},"reference-count":17,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2001,12,25]],"date-time":"2001-12-25T00:00:00Z","timestamp":1009238400000},"content-version":"vor","delay-in-days":603,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Tropical Med Int Health"],"published-print":{"date-parts":[[2000,5]]},"abstract":"<jats:p><jats:bold>Summary<\/jats:bold> We isolated <jats:italic>Acanthamoeba<\/jats:italic>e from the first two keratitis patients identified in Thailand in 1988 and 1990. The patients developed decreased vision, severe photophobia, severe eye pain and foreign body sensation after minor corneal trauma. The lesions included generalized superficial punctate keratitis, stromal corneal ulcer with keratic precipitate and uveitis in one case, and corneal ulcer with abscess in the other. Both cases were diagnosed by isolation of characteristic trophozoites and cysts of <jats:italic>Acanthamoeba<\/jats:italic> from corneal tissue by non\u2010nutrient agar culture method. Based on cyst morphology, <jats:italic>A. castellanii<\/jats:italic> and <jats:italic>A. polyphaga<\/jats:italic> were detected in one case, and <jats:italic>A. castellanii<\/jats:italic> and <jats:italic>A. triangularis<\/jats:italic> in the other. Restriction fragment length polymorphism analysis of mitochondrial DNA (mtDNA\u2010RFLP) revealed that each patient harboured a single parasite population. One shared mtDNA\u2010RFLP with an authentic strain of <jats:italic>A. castellanii<\/jats:italic>, and the other gave a new unique pattern. Thus species identification of <jats:italic>Acanthamoeba<\/jats:italic> based on cyst morphology <jats:italic>per se<\/jats:italic> can be arbitrary, and mtDNA\u2010RFLP may be more appropriate for accurate species\/strain differentiation amongst morphologically heterogeneous populations of <jats:italic>Acanthamoeba<\/jats:italic>e.<\/jats:p>","DOI":"10.1046\/j.1365-3156.2000.00563.x","type":"journal-article","created":{"date-parts":[[2003,3,12]],"date-time":"2003-03-12T21:34:45Z","timestamp":1047504885000},"page":"335-340","source":"Crossref","is-referenced-by-count":11,"title":["Heterogeneity in cyst morphology within isolates of <i>Acanthamoeba<\/i> from keratitis patients in Thailand"],"prefix":"10.1111","volume":"5","author":[{"given":"Somchai","family":"Jongwutiwes","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lalida","family":"Pariyakanok","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Malee","family":"Charoenkorn","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kenji","family":"Yagita","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takuro","family":"Endo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2001,12,25]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0161-6420(97)30092-X"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/clind\/13.Supplement_5.S403"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/eye.1990.132"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0161-6420(99)00515-1"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-5155(97)00109-3"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0163-4453(98)93054-7"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0161-6420(99)90169-0"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1136\/bjo.70.5.343"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9394(14)74321-1"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1093\/clind\/13.Supplement_5.S390"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/0035-9203(95)90429-8"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(05)64138-4"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-108-1-33"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.3109\/09273949709085064"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1095-8312.1985.tb02048.x"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1550-7408.1990.tb01267.x"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1007\/s004360050549"}],"container-title":["Tropical Medicine &amp; International Health"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1046%2Fj.1365-3156.2000.00563.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1046\/j.1365-3156.2000.00563.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,29]],"date-time":"2023-10-29T13:24:49Z","timestamp":1698585889000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1046\/j.1365-3156.2000.00563.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2000,5]]},"references-count":17,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2000,5]]}},"alternative-id":["10.1046\/j.1365-3156.2000.00563.x"],"URL":"https:\/\/doi.org\/10.1046\/j.1365-3156.2000.00563.x","archive":["Portico"],"relation":{},"ISSN":["1360-2276","1365-3156"],"issn-type":[{"value":"1360-2276","type":"print"},{"value":"1365-3156","type":"electronic"}],"subject":[],"published":{"date-parts":[[2000,5]]}}}