{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T20:52:45Z","timestamp":1789937565342,"version":"4.0.1"},"reference-count":26,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2001,12,25]],"date-time":"2001-12-25T00:00:00Z","timestamp":1009238400000},"content-version":"vor","delay-in-days":572,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Tropical Med Int Health"],"published-print":{"date-parts":[[2000,6]]},"abstract":"<jats:p><jats:bold>Summary<\/jats:bold> A comparative study of total saline extract (SE) and cyst vesicular fluid (VF) of <jats:italic>Taenia solium<\/jats:italic> metacestodes by ELISA and Western blotting assay (WB) tests was conducted to detect IgG in sera for diagnosis of human cysticercosis. Sera were obtained and analysed by ELISA in 1\u00a0:\u00a020 and 1\u00a0:\u00a0100 dilutions from 208 individuals: 22 confirmed neurocysticercosis (NC) (group 1), 101 suspected NC (group 2), 55 with various intestinal parasitosis (group 3) and 30 healthy individuals (group 4). The WB test was carried out on SE and VF extracts with and without reducing agent, 2\u2010\u03b2\u2010mercaptoethanol (2\u2010ME) in 20 sera of each group. WB using extracts without 2\u2010ME and ELISA at 1\u00a0:\u00a0100 dilution were compared in 20 sera from each group; sensitivity and specificity were calculated using samples from groups 1, 3 and 4. By ELISA, in the 1\u00a0:\u00a0100 sera dilution reactivity was reduced for both antigens without changes in the sensitivity of the test. By WB, antigens treated with 2\u2010ME demonstrated low specificity. For SE and VF antigens, the proteins of 24, 39\u201342, 47\u201352, 56, 64\u201368, 126\u2013155\u00a0kDa and 18, 24, 26\u201328, 32\u201336, 47\u201352, 75\u00a0kDa, respectively, were considered immunodominant markers, with high indices of specificity, suggesting a profile for NC patients. However, as the sensitivity was found to be low, it might still not be a definitive test for NC when used alone. These data suggest WB as an indicative test to determine exposure to <jats:italic>T. solium<\/jats:italic>. ELISA and WB together may supply reliable results for the diagnosis of human cysticercosis, since appropriate purified antigens are not available yet.<\/jats:p>","DOI":"10.1046\/j.1365-3156.2000.00567.x","type":"journal-article","created":{"date-parts":[[2003,3,12]],"date-time":"2003-03-12T21:34:57Z","timestamp":1047504897000},"page":"443-449","source":"Crossref","is-referenced-by-count":28,"title":["ELISA and Western Blotting tests in the detection of IgG antibodies to Taenia solium metacestodes in serum samples in human neurocysticercosis"],"prefix":"10.1111","volume":"5","author":[{"given":"Kely Yoshiko Martins","family":"Shiguekawa","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9 Roberto","family":"Mineo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leandro Pajuaba","family":"de Moura","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julia Maria","family":"Costa\u2010Cruz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2001,12,25]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-3156.1997.d01-379.x"},{"key":"e_1_2_6_3_1","first-page":"49","article-title":"A rea\u00e7\u00e3o imunoenzim\u00e1tica para cisticercose no l\u00edquido cefalorraquiano\u2010considera\u00e7\u00f5es sobre o limiar de reatividade.","volume":"27","author":"Bassi GE","year":"1991","journal-title":"Revista Brasileira de Patologia Cl\u00ednica"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1590\/S0004-282X1986000100002"},{"key":"e_1_2_6_5_1","first-page":"337","article-title":"Spinal fluid immunoenzimatic assay (ELISA) for neurocysticercosis.","volume":"24","author":"Costa JM","year":"1982","journal-title":"Revista do Instituto de Medicina Tropical de S\u00e3o Paulo"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1982.31.364"},{"key":"e_1_2_6_7_1","first-page":"61","volume-title":"Cisticercosis humana","author":"Flisser A","year":"1997"},{"key":"e_1_2_6_8_1","first-page":"27","article-title":"Human cysticercosis: antigens, antibodies and non\u2010responders.","volume":"39","author":"Flisser A","year":"1980","journal-title":"Clinical Experimental Immunology"},{"key":"e_1_2_6_9_1","first-page":"58","article-title":"Identification of immunodominant antigens by immunoelectrotransfer in hydatid fluid.","volume":"53","author":"Garc\u00eda A","year":"1998","journal-title":"Boletin Chileno de Parasitologia"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0035-9203(98)91069-0"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1986.35.308"},{"key":"e_1_2_6_12_1","first-page":"299","article-title":"Species\u2010specific immunodiagnosis of Taenia solium cysticercosis by ELISA and immunoblotting.","volume":"38","author":"Gottstein B","year":"1987","journal-title":"Tropical Medicine and Parasitology"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/0035-9203(93)90476-7"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1998.58.313"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/227680a0"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1989.40.282"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)52451-6"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1590\/S0074-02761919000100006"},{"key":"e_1_2_6_19_1","first-page":"773","article-title":"Determination of polisaccharide hexoses and hexosamines in normal human sera.","volume":"54","author":"Martirani I","year":"1959","journal-title":"Journal of Laboratory Clinical Medicine"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.2169\/internalmedicine.38.67"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1999.60.193"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00931618"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.76.9.4350"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/159.1.50"},{"key":"e_1_2_6_25_1","first-page":"215","volume-title":"Immunofluorescence and Related Techniques","author":"Wilson MB","year":"1978"},{"key":"e_1_2_6_26_1","first-page":"1004","article-title":"Significance of Western Blot in diagnosis of cysticercosis cellulosae","volume":"105","author":"Xu Z","year":"1992","journal-title":"Chinese Medical Journal"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1136\/jnnp.53.8.656"}],"container-title":["Tropical Medicine &amp; 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