{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T10:08:07Z","timestamp":1762078087349},"reference-count":35,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2008,7,7]],"date-time":"2008-07-07T00:00:00Z","timestamp":1215388800000},"content-version":"vor","delay-in-days":2775,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Tropical Med Int Health"],"published-print":{"date-parts":[[2000,12]]},"abstract":"<jats:p><jats:bold>Summary<\/jats:bold> The protein Ov20\/OvS1 was used as antigen in ELISA and Western blot in order to differentiate onchocerciasis from African mansonelliasis and to characterize the hyperreactive form of <jats:italic>Onchocerca volvulus<\/jats:italic> infection (sowda). The specificity of the IgG4 Western blot was 98% for the differentiation between persons with onchocerciasis and <jats:italic>Mansonella<\/jats:italic> microfilariae (mf) carriers (125 persons with <jats:italic>M. perstans<\/jats:italic> and 92 with <jats:italic>M. streptocerca<\/jats:italic>), whereas the IgG4 ELISA showed a specificity of 81% in 137\u00a0<jats:italic>M. perstans<\/jats:italic> mf carriers and 85% in 94\u00a0<jats:italic>M. streptocerca<\/jats:italic> mf carriers. The sensitivity of Ov20\/OvS1 in identifying onchocerciasis using the IgG4 ELISA was 75% for 103 <jats:italic>O. volvulus<\/jats:italic> mf carriers with the generalized and 89% for 44 patients with the sowda form of onchocerciasis. IgE antibodies against OvS1 were found in 95% of 39 patients with hyperreactive onchocerciasis but only in 15% of 47 persons with the generalized form. Thus, Ov20\/\u2013OvS1 appears a promising candidate antigen for the diagnosis of onchocerciasis and in particular for the detection of the sowda type of disease.<\/jats:p>","DOI":"10.1046\/j.1365-3156.2000.00655.x","type":"journal-article","created":{"date-parts":[[2003,3,12]],"date-time":"2003-03-12T21:34:00Z","timestamp":1047504840000},"page":"891-897","source":"Crossref","is-referenced-by-count":14,"title":["Use of the recombinant <i>Onchocerca volvulus<\/i> protein Ov20\/OvS1 for the immunodiagnostic differentiation between onchocerciasis and mansonelliasis and for the characterization of hyperreactive onchocerciasis (sowda)"],"prefix":"10.1111","volume":"5","author":[{"given":"J. L.","family":"Mpagi","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D. W.","family":"B\u00fcttner","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F. 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