{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T00:18:00Z","timestamp":1792801080899,"version":"4.5.0"},"reference-count":26,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2002,1,24]],"date-time":"2002-01-24T00:00:00Z","timestamp":1011830400000},"content-version":"vor","delay-in-days":23,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Tropical Med Int Health"],"published-print":{"date-parts":[[2002,1]]},"abstract":"<jats:p>Neurocysticercosis (NCC) is a major cause of morbidity and mortality in developed and developing countries. The diagnosis of this disease remains a problem. We report the detection of specific antigenic fraction (antigen B) of<jats:italic>Cysticercus cellulosae<\/jats:italic> by enzyme\u2010linked immunosorbent assay (ELISA) in various fractions of cerebrospinal fluid (CSF) obtained by high performance liquid chromatographic (HPLC) separation, for the diagnosis of human NCC. Forty patients attending or admitted to Nehru Hospital, Chandigarh were included in the study: 10 with suspected NCC, 20 with other neurological diseases and 10 undergoing surgery under spinal anaesthesia for non\u2010neurological conditions, who served as controls. CSF samples collected from all patients and controls were subjected to chromatographic separation on an HPLC system. Antigen B (AgB) was detected in separated fractions by an ELISA test and compared with the detection of antibody response in CSF samples by indirect haemagglutination (IHA) technique. Antigen B was detected in 9 out of 10 patients with suspected NCC based on clinical symptoms and radioimaging reports, but in none of the control subjects. However, antigen B was also detected in 9 out of 20 patients with other neurological disorders, mostly tubercular meningitis. Antibody response by IHA was found positive in only 2 of 10 cases clinically suspected of NCC. In conclusion, antigen B detection in CSF samples may be a useful adjunct to clinical suspicion and radiological reports for the diagnosis of NCC as there is no gold standard criteria to confirm this disease. However, the test needs to be evaluated on more patients in countries where tuberculosis and cysticercosis are endemic due to the high cross reactivity with samples from tubercular meningitis patients.<\/jats:p>","DOI":"10.1046\/j.1365-3156.2002.00810.x","type":"journal-article","created":{"date-parts":[[2003,3,12]],"date-time":"2003-03-12T21:37:46Z","timestamp":1047505066000},"page":"53-58","source":"Crossref","is-referenced-by-count":13,"title":["Detection of antigen B of <i>Cysticercus cellulosae<\/i> in cerebrospinal fluid for the diagnosis of human neurocysticercosis"],"prefix":"10.1111","volume":"7","author":[{"given":"Sanchita","family":"Das","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R. C.","family":"Mahajan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N. 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S.","family":"Sawhney","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Veena","family":"Dhawan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nancy","family":"Malla","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2002,1,24]]},"reference":[{"key":"e_1_2_5_2_2","doi-asserted-by":"publisher","DOI":"10.1055\/s-2008-1041117"},{"key":"e_1_2_5_3_2","doi-asserted-by":"publisher","DOI":"10.2307\/3282629"},{"key":"e_1_2_5_4_2","doi-asserted-by":"crossref","first-page":"364","DOI":"10.4269\/ajtmh.1982.31.364","article-title":"Enzyme linked immunosorbent assay (ELISA) for the detection of cysticerci of Taenia solium","volume":"31","author":"Diwan AR","year":"1982","journal-title":"American Journal of Tropical Medicine and Hygiene"},{"key":"e_1_2_5_5_2","doi-asserted-by":"crossref","first-page":"536","DOI":"10.1128\/jcm.24.4.536-541.1986","article-title":"Characterisation by ELISA of the humoral immune response in patients with neurocysticercosis and its application in immunodiagnosis","volume":"24","author":"Espinoza B","year":"1986","journal-title":"Journal of Clinical Microbiology"},{"key":"e_1_2_5_6_2","doi-asserted-by":"crossref","first-page":"50","DOI":"10.4269\/ajtmh.1989.41.50","article-title":"Identification of Taenia solium antigens in cerebrospinal fluid and larval antigens from patients with neurocysticercosis","volume":"41","author":"Estrada JJ","year":"1989","journal-title":"American Journal of Tropical Medicine and Hygiene"},{"key":"e_1_2_5_7_2","first-page":"27","article-title":"Human cysticercosis: antigen, antibody and nonresponders","volume":"39","author":"Flisser A","year":"1980","journal-title":"Clinical Experimental Immunology"},{"key":"e_1_2_5_8_2","doi-asserted-by":"publisher","DOI":"10.1136\/jnnp.62.6.612"},{"key":"e_1_2_5_9_2","doi-asserted-by":"publisher","DOI":"10.1007\/s004360050701"},{"key":"e_1_2_5_10_2","doi-asserted-by":"crossref","first-page":"605","DOI":"10.1080\/00034983.1991.11812616","article-title":"Comparative evaluation of cysticercal antigens and immunoassays in the diagnosis of neurocysticercosis","volume":"85","author":"Katti MK","year":"1991","journal-title":"American Journal of Tropical Medicine and Parasitology"},{"key":"e_1_2_5_11_2","first-page":"67","article-title":"Identification of antigenic fractions of Cysticercus cellulosae by western blotting in the serodiagnosis of human neurocysticercosis: before and after treatment","volume":"5","author":"Kaur M","year":"1995","journal-title":"Immunology and Infectious Diseases"},{"key":"e_1_2_5_12_2","first-page":"25","article-title":"Evaluation and characterization of purified antigenic fraction\u2010II of Cysticercus cellulosae by enzyme\u2010linked immunosorbent assay for the diagnosis of neurocysticercosis: before and after treatment","volume":"6","author":"Kaur M","year":"1996","journal-title":"Immunology and Infectious Diseases"},{"key":"e_1_2_5_13_2","doi-asserted-by":"publisher","DOI":"10.2307\/3283033"},{"key":"e_1_2_5_14_2","doi-asserted-by":"publisher","DOI":"10.1097\/00006123-199501000-00025"},{"key":"e_1_2_5_15_2","unstructured":"MahajanRC(1982)Geographical distribution of human cysticercosis. 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