{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T10:15:07Z","timestamp":1789208107117,"version":"build-2803163510"},"reference-count":24,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2002,4,22]],"date-time":"2002-04-22T00:00:00Z","timestamp":1019433600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["European Journal of Biochemistry"],"published-print":{"date-parts":[[2002,5]]},"abstract":"<jats:p>Phenylacetaldehyde reductase (PAR) produced by styrene\u2010assimilating <jats:italic>Corynebacterium<\/jats:italic> strain ST\u201010 was used to synthesize chiral alcohols. This enzyme with a broad substrate range reduced various prochiral aromatic ketones and \u03b2\u2010ketoesters to yield optically active secondary alcohols with an enantiomeric purity of more than 98% enantiomeric excess (e.e.). The <jats:italic>Escherichia coli<\/jats:italic> recombinant cells which expressed the <jats:italic>par<\/jats:italic> gene could efficiently produce important pharmaceutical intermediates; (<jats:italic>R<\/jats:italic>)\u20102\u2010chloro\u20101\u2010(3\u2010chlorophenyl)ethanol (28\u2003mg\u00b7mL<jats:sup>\u22121<\/jats:sup>) from <jats:italic>m<\/jats:italic>\u2010chlorophenacyl chloride, ethyl (<jats:italic>R<\/jats:italic>)\u20104\u2010chloro\u20103\u2010hydroxy butanoate) (28\u2003mg\u00b7mL<jats:sup>\u22121<\/jats:sup>) from ethyl 4\u2010chloro\u20103\u2010oxobutanoate and (<jats:italic>S<\/jats:italic>)\u2010<jats:italic>N\u2010tert<\/jats:italic>\u2010butoxycarbonyl(Boc)\u20103\u2010pyrrolidinol from <jats:italic>N<\/jats:italic>\u2010Boc\u20103\u2010pyrrolidinone (51\u2003mg\u00b7mL<jats:sup>\u22121<\/jats:sup>), with more than 86% yields. The high yields were due to the fact that PAR could concomitantly reproduce NADH in the presence of 3\u20137% (v\/v) 2\u2010propanol in the reaction mixture. This biocatalytic process provided one of the best asymmetric reductions ever reported.<\/jats:p>","DOI":"10.1046\/j.1432-1033.2002.02899.x","type":"journal-article","created":{"date-parts":[[2003,3,11]],"date-time":"2003-03-11T18:42:01Z","timestamp":1047408121000},"page":"2394-2402","source":"Crossref","is-referenced-by-count":83,"title":["Chiral alcohol production by NADH\u2010dependent phenylacetaldehyde reductase coupled with <i>in situ<\/i> regeneration of NADH"],"prefix":"10.1111","volume":"269","author":[{"given":"Nobuya","family":"Itoh","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michiko","family":"Matsuda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariko","family":"Mabuchi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tohru","family":"Dairi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jincun","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2002,4,24]]},"reference":[{"key":"e_1_2_7_2_2","volume-title":"Asymmetric Catalysis in Organic Synthesis","author":"Noyori R.","year":"1994"},{"key":"e_1_2_7_3_2","doi-asserted-by":"publisher","DOI":"10.1021\/ja00114a043"},{"key":"e_1_2_7_4_2","first-page":"233","article-title":"Reduction of 4\u2010substituted acetophenones by yeast","volume":"116","author":"Eichberger G.","year":"1985","journal-title":"Monatsh. 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