{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T09:34:12Z","timestamp":1791192852947,"version":"4.1.0"},"reference-count":63,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2003,1,13]],"date-time":"2003-01-13T00:00:00Z","timestamp":1042416000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["European Journal of Biochemistry"],"published-print":{"date-parts":[[2003,2]]},"abstract":"<jats:p>As human choriocarcinoma cells display many of the biochemical and morphological characteristics reported for <jats:italic>in utero<\/jats:italic> invasive trophoblast cells we have studied cholesterol supply from high density lipoproteins (HDL) to these cells. Binding properties of <jats:sup>125<\/jats:sup>I\u2010labeled HDL subclass 3 (HDL<jats:sub>3<\/jats:sub>) at 4\u2003\u00b0C were similar for BeWo, JAr, and Jeg3 choriocarcinoma cell lines while degradation rates at 37\u2003\u00b0C were highest for BeWo. Calculating the selective cholesteryl ester (CE)\u2010uptake as the difference between specific cell association of [<jats:sup>3<\/jats:sup>H]CE\u2010labeled HDL<jats:sub>3<\/jats:sub> and holoparticle association of <jats:sup>125<\/jats:sup>I\u2010labeled HDL<jats:sub>3<\/jats:sub> revealed that in BeWo cells, the selective CE\u2010uptake was slightly lower than holoparticle association. However, the pronounced capacity for specific cell association of [<jats:sup>3<\/jats:sup>H]CE\u2010HDL<jats:sub>3<\/jats:sub> and selective [<jats:sup>3<\/jats:sup>H]CE\u2010uptake in excess of HDL<jats:sub>3<\/jats:sub>\u2013holoparticle association, and cAMP\u2013mediated enhanced cell association of [<jats:sup>3<\/jats:sup>H]CE\u2010HDL<jats:sub>3<\/jats:sub> in JAr and Jeg3 suggested the scavenger receptor class B, type I (SR\u2010BI) to be responsible for this pathway. Abundant expression of SR\u2010BI (but not SR\u2010BII, a splice variant of SR\u2010BI) could be observed in JAr and Jeg3 but not in BeWo cells using RT\u2010PCR, Northern and Western blot analysis, and immunocytochemical technique. Adenovirus\u2010mediated overexpression of SR\u2010BI in all three choriocarcinoma cell lines resulted in an enhanced capacity for cell association of [<jats:sup>3<\/jats:sup>H]CE\u2010HDL<jats:sub>3<\/jats:sub> (20\u2010fold in BeWo; fivefold in JAr and Jeg3). The fact that exogenous HDL<jats:sub>3<\/jats:sub> remarkably increases proliferation in JAr and Jeg3 supports the notion that selective CE\u2010uptake and subsequent intracellular generation of cholesterol is coupled to cellular growth. From our findings we propose that JAr and Jeg3 cells serve as a suitable <jats:italic>in vitro<\/jats:italic> model to study selective CE\u2010supply to human placental cells.<\/jats:p>","DOI":"10.1046\/j.1432-1033.2003.03394.x","type":"journal-article","created":{"date-parts":[[2003,3,11]],"date-time":"2003-03-11T18:43:56Z","timestamp":1047408236000},"page":"451-462","source":"Crossref","is-referenced-by-count":44,"title":["Trophoblast\u2010like human choriocarcinoma cells serve as a suitable <i>in vitro<\/i> model for selective cholesteryl ester uptake from high density lipoproteins"],"prefix":"10.1111","volume":"270","author":[{"given":"Christian","family":"Wadsack","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andelko","family":"Hrzenjak","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Astrid","family":"Hammer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Birgit","family":"Hirschmugl","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sanja","family":"Levak\u2010Frank","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gernot","family":"Desoye","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wolfgang","family":"Sattler","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ernst","family":"Malle","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2003,1,13]]},"reference":[{"key":"e_1_2_27_2_2","doi-asserted-by":"publisher","DOI":"10.1097\/00041433-200106000-00010"},{"key":"e_1_2_27_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/0303-7207(84)90118-7"},{"key":"e_1_2_27_4_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00496817"},{"key":"e_1_2_27_5_2","doi-asserted-by":"publisher","DOI":"10.1210\/jcem-54-5-903"},{"key":"e_1_2_27_6_2","doi-asserted-by":"crossref","first-page":"1246","DOI":"10.1016\/S0022-2275(20)39154-9","article-title":"Origin of cholesterol in the fetal golden Syrian hamster: contribution of de novo sterol synthesis and maternal\u2010derived lipoprotein cholesterol","volume":"37","author":"Woollett L.A.","year":"1996","journal-title":"J. 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