{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T20:46:11Z","timestamp":1792097171402,"version":"4.4.0"},"reference-count":0,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2001,12,25]],"date-time":"2001-12-25T00:00:00Z","timestamp":1009238400000},"content-version":"vor","delay-in-days":1289,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["European Journal of Biochemistry"],"published-print":{"date-parts":[[1998,6,15]]},"abstract":"<jats:p>Potassium\u2010channel\u2010blocking scorpion toxins (\u03b1\u2010K\u2010toxins) have been shown to be valuable tools for the study of potassium channels. Here we report two toxins, cobatoxin 1 and 2, of 32 amino acids, containing three disulphide bridges, that were isolated from the venom of the Mexican scorpion <jats:italic>Centruroides noxius<\/jats:italic>. Their primary sequences show less than 40\u2003% identity to other \u03b1\u2010K\u2010toxins. It is therefore proposed that they belong to subfamily 9. The cDNA of cobatoxin 1 encodes a putative signal peptide, a putative short propeptide, the mature peptide and two amino acids that are processed to leave cobatoxin 1 amidated at the C\u2010terminus. In rat brain synaptosomal membranes cobatoxin 1 and cobatoxin 2 bind to a common binding site of \u03b1\u2010K\u2010toxins with <jats:italic>K<\/jats:italic><jats:sub>i<\/jats:sub> values of 109\u2004pM and 87\u2004pM, respectively. Moreover, they block the Shaker and K<jats:sub>V<\/jats:sub>1.1 K<jats:sup>+<\/jats:sup> channels with moderate affinities, with <jats:italic>K<\/jats:italic><jats:sub>d<\/jats:sub> values of around 0.7\u2004\u03bcM and 4.1\u2004\u03bcM (Shaker) and 0.5\u2004\u03bcM and 1.0\u2004\u03bcM (K<jats:sub>V<\/jats:sub>1.1), respectively. A three\u2010dimensional model of cobatoxin 1 was generated and used to interpret the obtained functional data on a structural basis.<\/jats:p>","DOI":"10.1046\/j.1432-1327.1998.2540468.x","type":"journal-article","created":{"date-parts":[[2003,3,11]],"date-time":"2003-03-11T17:01:01Z","timestamp":1047402061000},"page":"468-479","source":"Crossref","is-referenced-by-count":32,"title":["Cobatoxins 1 and 2 from <i>Centruroides noxius<\/i> Hoffmann constitute a subfamily of potassium\u2010channel\u2010blocking scorpion toxins"],"prefix":"10.1111","volume":"254","author":[{"given":"Barbara","family":"Selisko","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Consuelo","family":"Garcia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Baltazar","family":"Becerril","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Froylan","family":"G\u00f3mez\u2010Lagunas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlos","family":"Garay","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lourival D.","family":"Possani","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2001,12,25]]},"container-title":["European Journal of Biochemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1046%2Fj.1432-1327.1998.2540468.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/pdf\/10.1046\/j.1432-1327.1998.2540468.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,23]],"date-time":"2023-11-23T06:33:27Z","timestamp":1700721207000},"score":1,"resource":{"primary":{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/10.1046\/j.1432-1327.1998.2540468.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1998,6,15]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1998,6,15]]}},"alternative-id":["10.1046\/j.1432-1327.1998.2540468.x"],"URL":"https:\/\/doi.org\/10.1046\/j.1432-1327.1998.2540468.x","archive":["Portico"],"relation":{},"ISSN":["0014-2956","1432-1033"],"issn-type":[{"value":"0014-2956","type":"print"},{"value":"1432-1033","type":"electronic"}],"subject":[],"published":{"date-parts":[[1998,6,15]]}}}