{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T16:30:32Z","timestamp":1790267432526,"version":"4.1.0"},"reference-count":30,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2001,12,25]],"date-time":"2001-12-25T00:00:00Z","timestamp":1009238400000},"content-version":"vor","delay-in-days":603,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["European Journal of Biochemistry"],"published-print":{"date-parts":[[2000,5]]},"abstract":"<jats:p>A defined medium with ethanol as sole carbon source was devised for growth of the colorless, unicellular alga <jats:italic>Polytomella<\/jats:italic> spp. Cell density on this carbon source was related to extracellular pH. An acidic pH was required for ethanol utilization; best yields were obtained at pH\u20033.7. Spectroscopic analysis of the cells showed that the concentration of cytochrome <jats:italic>c<\/jats:italic> per cell was 40% higher than at pH\u20036.0; the concentrations of cytochrome <jats:italic>a<\/jats:italic><jats:sub>606<\/jats:sub> (cytochrome <jats:italic>c<\/jats:italic> oxidase) and <jats:italic>b<\/jats:italic><jats:sub>566<\/jats:sub> (cytochrome <jats:italic>bc<\/jats:italic><jats:sub>1<\/jats:sub> complex) were the same. A soluble cytochrome <jats:italic>c<\/jats:italic><jats:sub>550<\/jats:sub> was purified from cells grown at pH 3.7 and characterized by peptide sequencing as the 12\u2010kDa cytochrome <jats:italic>c<\/jats:italic><jats:sub>550<\/jats:sub> of the mitochondrial respiratory chain. Immunoblots of total cell proteins showed higher accumulation of cytochrome <jats:italic>c<\/jats:italic><jats:sub>550<\/jats:sub> at pH\u20033.7 than at pH\u20036.0. RNA blot analysis gave clear evidence of the abundance of <jats:italic>c<\/jats:italic><jats:sub>550<\/jats:sub> transcript in cells grown at pH\u20033.7. The amount of mitochondrial proteins obtained from cells grown at pH\u20033.7 was twofold higher than that of cells grown at pH\u20036.0. Mitochondria isolated from both cell types readily oxidized succinate, malate or ethanol. The rates of oxygen uptake were 20\u201325% higher in mitochondria from cells grown at pH\u20033.7. Cyanide and antimycin\u2003A inhibited respiration with succinate up to 95% in both types of mitochondria. The participation of cytochrome <jats:italic>c<\/jats:italic><jats:sub>550<\/jats:sub> in mitochondrial electron transport from succinate to oxygen was shown by spectral measurements.<\/jats:p>","DOI":"10.1046\/j.1432-1327.2000.01288.x","type":"journal-article","created":{"date-parts":[[2003,3,11]],"date-time":"2003-03-11T17:45:29Z","timestamp":1047404729000},"page":"2850-2858","source":"Crossref","is-referenced-by-count":17,"title":["<i>Polytomella<\/i> spp. growth on ethananol"],"prefix":"10.1111","volume":"267","author":[{"given":"Ariane","family":"Atteia","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert","family":"van Lis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jorge","family":"Ram\u00edrez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diego","family":"Gonz\u00e1lez\u2010Halphen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2001,12,25]]},"reference":[{"key":"e_1_2_7_2_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1550-7408.1955.tb02413.x"},{"key":"e_1_2_7_3_2","first-page":"608","volume-title":"Handbook of Protoctista","author":"Melkonian M.","year":"1990"},{"key":"e_1_2_7_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/0303-2647(80)90038-6"},{"key":"e_1_2_7_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2728(95)00158-1"},{"key":"e_1_2_7_6_2","doi-asserted-by":"publisher","DOI":"10.1006\/abbi.1998.0680"},{"key":"e_1_2_7_7_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00027073"},{"key":"e_1_2_7_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0005-2728(97)00031-5"},{"key":"e_1_2_7_9_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1550-7408.1955.tb02416.x"},{"key":"e_1_2_7_10_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1550-7408.1959.tb03921.x"},{"key":"e_1_2_7_11_2","first-page":"407","article-title":"Limites de concentrations en ions H+ et OH\u2212 compatibles avec le d\u00e9veloppement in vitro du flagell\u00e9Polytomella caeca","volume":"66","author":"Lwoff A.","year":"1941","journal-title":"Annales de l\u2019Institut Pasteur"},{"key":"e_1_2_7_12_2","volume-title":"The Chlamydomonas Sourcebook: a Comprehensive Guide to Biology and Laboratory Use.","author":"Harris E.H.","year":"1980"},{"key":"e_1_2_7_13_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1550-7408.1975.tb00960.x"},{"key":"e_1_2_7_14_2","doi-asserted-by":"crossref","first-page":"226","DOI":"10.1016\/S0021-9258(18)48483-9","article-title":"Identification of mitochondrial proteins in membrane preparations from Chlamydomonas reinhardtii","volume":"267","author":"Atteia A.","year":"1992","journal-title":"J. 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