{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T09:07:30Z","timestamp":1791104850066,"version":"4.1.0"},"reference-count":34,"publisher":"Wiley","issue":"17","license":[{"start":{"date-parts":[[2003,10,3]],"date-time":"2003-10-03T00:00:00Z","timestamp":1065139200000},"content-version":"vor","delay-in-days":1127,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["European Journal of Biochemistry"],"published-print":{"date-parts":[[2000,9]]},"abstract":"<jats:p>Two toxin\u2010like proteins (AahTL1 and AahTL3) were purified from the venom of the scorpion <jats:italic>Androctonus australis<\/jats:italic> Hector (Aah). AahTL1 and AahTL3 are the first non toxic proteins cross\u2010reacting with AahI toxins group which indicates that these proteins can be used as a model of vaccins.<\/jats:p><jats:p>In order to study structure\u2010function relationships, their complete amino\u2010acid sequences (66 residues) were determined, by automated Edman degradation. They show more than 50% of similarity with both AahI and AahIII antimammal toxins. Three\u2010dimensional structural models of AahTL1 and AahTL3 constructed by homology suggest that the two proteins are structurally similar to antimammal scorpion \u03b1\u2010toxins specific to voltage dependent Na<jats:sup>+<\/jats:sup> channels. The models showed also that amino\u2010acid changes between potent Aah toxins and both AahTL1 and AahTL3 disrupt the electrostatic potential gradient at their surface preventing their interaction with the receptor, which may explain their non toxicity.<\/jats:p>","DOI":"10.1046\/j.1432-1327.2000.01632.x","type":"journal-article","created":{"date-parts":[[2003,3,11]],"date-time":"2003-03-11T17:52:29Z","timestamp":1047405149000},"page":"5614-5620","source":"Crossref","is-referenced-by-count":22,"title":["Purification, characterization and molecular modelling of two toxin\u2010like proteins from the <i>Androctonus australis<\/i> Hector venom"],"prefix":"10.1111","volume":"267","author":[{"given":"Najet","family":"Srairi\u2010Abid","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pascal","family":"Mansuelle","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thouraya","family":"Mejri","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Habib","family":"Karoui","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Herv\u00e9","family":"Rochat","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fran\u00e7ois","family":"Sampieri","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohamed","family":"El Ayeb","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2003,10,3]]},"reference":[{"key":"e_1_2_7_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/0041-0101(95)00130-1"},{"key":"e_1_2_7_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/0041-0101(88)90215-2"},{"key":"e_1_2_7_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/0041-0101(96)83728-7"},{"key":"e_1_2_7_5_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1996.0653w.x"},{"key":"e_1_2_7_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/s0264-410x(97)00144-8"},{"key":"e_1_2_7_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/0041-0101(83)90276-3"},{"key":"e_1_2_7_8_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1970.tb01111.x"},{"key":"e_1_2_7_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/0041-0101(81)90044-1"},{"key":"e_1_2_7_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/0264-410X(91)90012-U"},{"key":"e_1_2_7_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/s0968-0004(98)01285-7"},{"key":"e_1_2_7_12_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.85.20.7443"},{"key":"e_1_2_7_13_2","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.1995.0233"},{"key":"e_1_2_7_14_2","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.1993.1626"},{"key":"e_1_2_7_15_2","doi-asserted-by":"publisher","DOI":"10.1107\/S0021889892009944"},{"key":"e_1_2_7_16_2","doi-asserted-by":"publisher","DOI":"10.1002\/prot.340170404"},{"key":"e_1_2_7_17_2","doi-asserted-by":"publisher","DOI":"10.1002\/prot.340110407"},{"key":"e_1_2_7_18_2","doi-asserted-by":"crossref","first-page":"2602","DOI":"10.1016\/S0021-9258(19)74315-4","article-title":"Synthesis and characterization of kaliotoxin. 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