{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,30]],"date-time":"2023-10-30T08:43:04Z","timestamp":1698655384298},"reference-count":15,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2001,12,25]],"date-time":"2001-12-25T00:00:00Z","timestamp":1009238400000},"content-version":"vor","delay-in-days":603,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Veterinary Medicine, Series B"],"published-print":{"date-parts":[[2000,5]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>The first cases of scrapie were detected in Greece in a flock of sheep in October 1986. All the animals of the affected flock and all sheep in two flocks that were in contact were killed and buried. A systematic investigation of all available cases with signs indicating a neurological disease started in sheep and goats in late 1986, as well as in cattle in 1989. The investigation was based on clinical examination, necropsy or macroscopical examination of the brain and viscera, and histological examination of the brain in all animals except those with coenurosis. Histological examinations of specimens from the spinal cord and other tissues, and if considered necessary bacteriological, toxicological and serological examinations were also carried out. In October 1997, scrapie was diagnosed in sheep of a second flock (a mixed flock of sheep and goats), grazing in a pasture close to the place where scrapie was initially detected. All animals of the second flock were also killed and buried. Diagnosis in the first flock was based on clinical signs and histological lesions, and in the second immunoblotting was also used. Distinctive lesions of scrapie were found in the brain and\/or the spinal cord of eight sheep with clinical signs from the two flocks. The lesions were revealed in the brain stem and\/or in the cervical spinal cord, and tended to be symmetrical. In one sheep, severe lesions in the cortex of cerebral hemispheres and of the cerebellum were also found. In the brain of two sheep from the second flock the pathological isoform of PrP protein was detected. Despite the eradication scheme applied, scrapie in sheep reappeared after 11 years in a place close to where it occurred initially. This may indicate that the effectiveness of the eradication scheme implemented was not adequate and additional approaches may be needed.<\/jats:p>","DOI":"10.1046\/j.1439-0450.2000.00348.x","type":"journal-article","created":{"date-parts":[[2003,3,12]],"date-time":"2003-03-12T09:13:11Z","timestamp":1047460391000},"page":"303-309","source":"Crossref","is-referenced-by-count":12,"title":["A Survey of More Than 11 Years of Neurologic Diseases of Ruminants with Special Reference to Transmissible Spongiform Encephalopathies (TSEs) in Greece"],"prefix":"10.1111","volume":"47","author":[{"given":"S.","family":"Leontides","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"V.","family":"Psychas","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Aargyroudis","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Giannati\u2010stefanou","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E.","family":"Paschaleri\u2010papadopoulou","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T.","family":"Manousis","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T.","family":"Sklaviadis","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2001,12,25]]},"reference":[{"key":"e_1_2_6_2_1","first-page":"970","volume-title":"The Merck Veterinary Manual","author":"Aiello S. E.","year":"1998"},{"key":"e_1_2_6_3_1","first-page":"141","volume-title":"The first cases of scrapie in a flock of sheep in Greece","author":"Argyroudis S.","year":"1987"},{"key":"e_1_2_6_4_1","unstructured":"CEC 1994:Transmissible Spongiform Encephalopathies. Protocols for the Laboratory Diagnosis and Confirmation of Bovine Spongiform Encephalopathy and Scrapie.A Protocol from the Scientific Veterinary Committee September 1994. European Commission."},{"key":"e_1_2_6_5_1","first-page":"380","volume-title":"Pathology of Domestic Animals","author":"Jubb K. V. E.","year":"1993"},{"key":"e_1_2_6_6_1","first-page":"71","volume-title":"Diseases of Sheep","author":"Mitchell B.","year":"1983"},{"key":"e_1_2_6_7_1","first-page":"661","article-title":"Fifty years with scrapie: A personal reminiscence","volume":"23","author":"Pattison H. I.","year":"1988","journal-title":"Vet. Rec."},{"key":"e_1_2_6_8_1","first-page":"1112","volume-title":"Veterinary Medicine","author":"Radostits O. M.","year":"1994"},{"key":"e_1_2_6_9_1","doi-asserted-by":"crossref","first-page":"1212","DOI":"10.1128\/jvi.63.3.1212-1222.1989","article-title":"Physical properties of the Creutzfeldt\u2010Jakob disease infectious agent","volume":"63","author":"Sklaviadis T.","year":"1989","journal-title":"J. Virol"},{"key":"e_1_2_6_10_1","first-page":"133","volume-title":"Goat Medicine","author":"Smith M. C.","year":"1994"},{"key":"e_1_2_6_11_1","first-page":"376","volume-title":"Tomson's Special Veterinary Pathology","author":"Storts R. W.","year":"1995"},{"key":"e_1_2_6_12_1","first-page":"136","volume-title":"Veterinary Neuropathology","author":"Summers B. A.","year":"1995"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/0007-1935(88)90157-1"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/0007-1935(91)90105-V"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1136\/vr.140.7.167"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0368-1742(58)80016-8"}],"container-title":["Journal of Veterinary Medicine, Series B"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1046%2Fj.1439-0450.2000.00348.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1046\/j.1439-0450.2000.00348.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,29]],"date-time":"2023-10-29T16:19:00Z","timestamp":1698596340000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1046\/j.1439-0450.2000.00348.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2000,5]]},"references-count":15,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2000,5]]}},"alternative-id":["10.1046\/j.1439-0450.2000.00348.x"],"URL":"https:\/\/doi.org\/10.1046\/j.1439-0450.2000.00348.x","archive":["Portico"],"relation":{},"ISSN":["0931-1793"],"issn-type":[{"value":"0931-1793","type":"print"}],"subject":[],"published":{"date-parts":[[2000,5]]}}}