{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T22:09:23Z","timestamp":1787004563597,"version":"build-2736575974"},"reference-count":26,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2002,4,20]],"date-time":"2002-04-20T00:00:00Z","timestamp":1019260800000},"content-version":"vor","delay-in-days":50,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Veterinary Medicine, Series B"],"published-print":{"date-parts":[[2002,3]]},"abstract":"<jats:p>\n                    Rotaviruses are important pathogens associated with diarrhoeal diseases in almost all species of mammals. In the present study, a nested reverse transcriptase\u2010polymerase chain reaction (RT\u2010PCR) for the detection of group A rotaviruses was developed, which is based on a target region in gene segment 6. Rotavirus strains of human, bovine, porcine, canine, feline, equine, and ovine origin were examined. Furthermore several faecal specimens, in which rotavirus had already been detected using other methods than PCR, were included in the study. A nested RT\u2010PCR product was formed with all strains and faecal samples tested. The detection limit for virus\u2010containing cell culture supernatant was 3\u2003\u00d7\u200310\n                    <jats:sup>\u20132<\/jats:sup>\n                    [50% tissue culture infective dose (TCID\n                    <jats:sub>50<\/jats:sub>\n                    )] by RT\u2010PCR and 3\u2003\u00d7\u200310\n                    <jats:sup>\u20133<\/jats:sup>\n                    TCID\n                    <jats:sub>50<\/jats:sub>\n                    by nested amplification. In order to examine the influence of the sample matrix on sensitivity, a rotavirus\u2010negative faecal specimen was spiked with virus\u2010containing cell culture suspension of the porcine rotavirus OSU. The detection limit of the present PCR procedure was approximately 1.6\u2003\u00d7\u200310\n                    <jats:sup>2<\/jats:sup>\n                    TCID\n                    <jats:sub>50<\/jats:sub>\n                    per g faeces and could be increased by one order of magnitude using nested PCR. The present method for detection and identification of group A rotaviruses represents a powerful diagnostic tool and was shown to be applicable to rotaviruses of different origin, including human sources.\n                  <\/jats:p>","DOI":"10.1046\/j.1439-0450.2002.00510.x","type":"journal-article","created":{"date-parts":[[2003,3,12]],"date-time":"2003-03-12T04:15:25Z","timestamp":1047442525000},"page":"77-81","source":"Crossref","is-referenced-by-count":65,"title":["Nested Reverse Transcriptase\u2010Polymerase Chain Reaction for the Detection of Group A Rotaviruses"],"prefix":"10.1111","volume":"49","author":[{"given":"M.","family":"ELSCHNER","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"PRUDLO","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H.","family":"HOTZEL","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.","family":"OTTO","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K.","family":"SACHSE","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2002,4,20]]},"reference":[{"key":"e_1_2_6_2_2","first-page":"825","article-title":"Isolation and identification of rotaviruses as cause of diarrhoea in piglets and their distribution in pigs in Germany","volume":"34","author":"Bachmann P. 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