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To test for the presence of the virus, latex (On Site Biotech, Uppsala, Sweden) and direct immunofluorescence tests were performed. At the same time, polymerase chain reaction (PCR) with primers complementary to a conservative region of VP1\/VP2 was carried out. The products of amplification were analysed on 2% agarose gel. The purified products were cloned with the Template Generation System (Finnzymes, Espoo, Finland) using a transposition reaction and positive clones were searched using the `colony screening by PCR' method. The sequencing gave 12 sequences of VP1\/VP2 gene fragments that were of high similarity. Among the 12 analysed sequences, six exhibited 88% similarity, four exhibited 100% similarity and two exhibited 71% similarity.<\/jats:p>","DOI":"10.1046\/j.1439-0450.2002.00523.x","type":"journal-article","created":{"date-parts":[[2003,3,12]],"date-time":"2003-03-12T09:15:52Z","timestamp":1047460552000},"page":"142-145","source":"Crossref","is-referenced-by-count":3,"title":["Phylogenetic Similarity of the Canine Parvovirus Wild\u2010Type Isolates on the Basis of VP1\/VP2 Gene Fragment Sequence Analysis"],"prefix":"10.1111","volume":"49","author":[{"given":"K.","family":"RYPU\u0141A","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R.","family":"CHMIELEWSKI","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E.","family":"\u015aMIELEWSKA\u2010\u0141O\u015a","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"KLIMENTOWSKI","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2002,5,2]]},"reference":[{"key":"e_1_2_5_2_2","first-page":"1516","article-title":"Status report: canine viral enteritis","volume":"173","author":"Appel M. 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