{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,26]],"date-time":"2025-05-26T11:43:45Z","timestamp":1748259825664},"reference-count":32,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2002,5,2]],"date-time":"2002-05-02T00:00:00Z","timestamp":1020297600000},"content-version":"vor","delay-in-days":31,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Veterinary Medicine, Series B"],"published-print":{"date-parts":[[2002,4]]},"abstract":"<jats:p>In this study, a dot\u2010enzyme\u2010linked immunosorbent assay (Dot\u2010ELISA) was evaluated in comparison with a complement fixation test (CFT) for the detection of<jats:italic>Campylobacter<\/jats:italic>antibodies in sheep sera. Acid glycine extracts (AGE) of both<jats:italic>Campylobacter fetus<\/jats:italic>ssp.<jats:italic>fetus<\/jats:italic>and<jats:italic>Campylobacter jejuni<\/jats:italic>strains that had been isolated from the gall\u2010bladder of slaughtered sheep was used as antigen in both tests. A total of 153 sheep sera from aborted (74) and slaughtered (79) sheep were examined by both Dot\u2010ELISA and CFT. Twenty\u2010two sera showed anti\u2010complementary activity were not suitable for CFT. Of the 22 sera showing anti\u2010complementary activity, two sera were found to be positive in Dot\u2010ELISA. Eighty\u2010eight (67.2%) of the remaining 131 sera were negative by both Dot\u2010ELISA and CFT using AGE of both<jats:italic>Campylobacter<\/jats:italic>strains whereas 43 sera (32.8%) gave different reaction patterns in Dot\u2010ELISA and CFT with the extracts of both<jats:italic>Campylobacter<\/jats:italic>strains. Twelve sera were positive by both tests using AGE of<jats:italic>C. fetus<\/jats:italic>ssp.<jats:italic>fetus<\/jats:italic>but CFT failed to detect antibodies in nine of these sera when AGE of<jats:italic>C. jejuni<\/jats:italic>was used. Twelve sera were positive by both tests only when AGE of<jats:italic>C. fetus<\/jats:italic>ssp.<jats:italic>fetus<\/jats:italic>was used. Eleven sera were positive only by CFT. Seven of these reacted only with the AGE of C<jats:italic>. fetus<\/jats:italic>ssp.<jats:italic>fetus<\/jats:italic>and four sera were positive by using AGE of both<jats:italic>Campylobacter<\/jats:italic>strains. The remaining eight sera were found to be positive only by dot\u2010immunobinding assay either with the AGE of both<jats:italic>Campylobacter<\/jats:italic>strains or with the AGE of one of the<jats:italic>Campylobacter<\/jats:italic>strains. It is concluded that Dot\u2010ELISA using AGE from<jats:italic>C. fetus<\/jats:italic>ssp.<jats:italic>fetus<\/jats:italic>could be employed for the detection of<jats:italic>Campylobacter<\/jats:italic>antibodies in sheep sera and the additional use of AGE from<jats:italic>C. jejuni<\/jats:italic>as antigen appeared not to be profitable for this purpose.<\/jats:p>","DOI":"10.1046\/j.1439-0450.2002.00524.x","type":"journal-article","created":{"date-parts":[[2003,3,12]],"date-time":"2003-03-12T09:15:52Z","timestamp":1047460552000},"page":"146-151","source":"Crossref","is-referenced-by-count":1,"title":["Detection of<i>Campylobacter<\/i>Antibodies in Sheep Sera by a Dot\u2010ELISA Using Acid Extracts from<i>C. fetus<\/i>ssp.<i>fetus<\/i>and<i>C. jejuni<\/i>Strains and Comparison with a Complement Fixation Test"],"prefix":"10.1111","volume":"49","author":[{"given":"K.","family":"G\u00dcRT\u00dcRK","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"\u0130. H.","family":"EK\u0130N","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"AKSAKAL","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H.","family":"SOLMAZ","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2002,5,2]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1135(92)90139-K"},{"key":"e_1_2_6_3_2","unstructured":"Alton G. G. M. L.Jones D. D.Angus andJ. M.Verger 1988: Techniques for the Brucellosis Laboratory. INRA Ed Paris France."},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1135(89)90038-2"},{"key":"e_1_2_6_5_2","unstructured":"Blobel H.andT.Schliesser 1982: Handbuch der bakteriellen Infektionen bei Tieren. VEB Gustav. Fisher Verlag Jena Germany."},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2249.2000.01349.x"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1135(89)90052-7"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0450.1989.tb00613.x"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2672.1992.tb01860.x"},{"key":"e_1_2_6_10_2","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1186\/BF03546562","article-title":"Detection of Campylobacter antibodies and its application in seroepidemiological studies in sheep and cattle herds","volume":"26","author":"Gr\u00f6hn K.","year":"1985","journal-title":"Acta Vet. Scand."},{"key":"e_1_2_6_11_2","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1111\/j.0931-1793.1999.0_273.x","article-title":"Comparison of the dot\u2010immunobinding assay with the serum agglutination test, the Rose Bengal plate test and the milk ring test for the detection of Brucella antibodies in bovine sera and milk","volume":"46","author":"G\u00fcrt\u00fcrk K.","year":"1999","journal-title":"J. Vet. Med. B"},{"key":"e_1_2_6_12_2","doi-asserted-by":"crossref","first-page":"341","DOI":"10.55730\/1300-0128.4018","article-title":"Detection of Brucella antibodies in sheep sera using dot\u2010immunobinding assay and Rose Bengal plate agglutination test","volume":"21","author":"G\u00fcrt\u00fcrk K.","year":"1997","journal-title":"Tr. J. Vet. Anim Sci., T\u00dcB\u0130TAK"},{"key":"e_1_2_6_13_2","unstructured":"Halliwell R. E. W. andN. T.Gorman 1989: Veterinary Clinical Immunology. W.B. Saunders Company Philadelphia PA USA."},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(82)90677-7"},{"key":"e_1_2_6_15_2","doi-asserted-by":"crossref","first-page":"1262","DOI":"10.1128\/jcm.25.7.1262-1264.1987","article-title":"Serodiagnosis of rabies by dot\u2010immunobinding assay","volume":"25","author":"Heberling R. L.","year":"1987","journal-title":"J. Clin. Microbiol."},{"key":"e_1_2_6_16_2","unstructured":"Holt J. G. N. R.Krieg P. H. A.Sneath J. T.Staley andS. T.Williams 1994: Bergey's Manuel of Determinative Bacteriology Ninth Edition. 1994. Williams & Wilkins Baltimore MD USA"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1751-0813.1991.tb03240.x"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.33.8.767"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01641193"},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/0022-1759(85)90065-1"},{"key":"e_1_2_6_21_2","doi-asserted-by":"crossref","first-page":"675","DOI":"10.1128\/iai.42.2.675-682.1983","article-title":"Molecular identification of surface protein antigens of Campylobacter jejuni","volume":"42","author":"Logan S. M.","year":"1983","journal-title":"Infect. Immun."},{"key":"e_1_2_6_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.11.3.517-525.1975"},{"key":"e_1_2_6_23_2","first-page":"29","article-title":"Antibody response in patients infected with Campylobacter jejuni\/coli assayed with a complement fixation test and a DIG\u2010ELISA method","volume":"10","author":"Melby K.","year":"1987","journal-title":"NIPH Ann."},{"key":"e_1_2_6_24_2","first-page":"846","article-title":"Serologische diagnose menschlicher Campylobacter Infektionen","author":"Mosimann J.","year":"1981","journal-title":"Schweiz. Med. Wochenschr."},{"key":"e_1_2_6_25_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF02439941"},{"key":"e_1_2_6_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1135(93)90105-G"},{"key":"e_1_2_6_27_2","first-page":"103","article-title":"Acid and alkaline Campylobacter extracts in the demonstration of antibody response in hyperimmunized rabbits","volume":"95","author":"Rautelin H.","year":"1987","journal-title":"Acta Path. Microbiol. Immunol. Scand. Sect. B"},{"key":"e_1_2_6_28_2","doi-asserted-by":"crossref","first-page":"700","DOI":"10.1128\/jcm.17.4.700-701.1983","article-title":"An acid extract as a common antigen in Campylobacter coli and Campylobacter jejuni strains","volume":"17","author":"Rautelin H.","year":"1983","journal-title":"J. Clin. Microbiol."},{"key":"e_1_2_6_29_2","first-page":"277","article-title":"Single and multiple acid extract antigens in Campylobacter serology","volume":"95","author":"Rautelin H.","year":"1987","journal-title":"Acta Path. Microbiol. Immunol. Scand. Sect. B"},{"key":"e_1_2_6_30_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/148.1.82"},{"key":"e_1_2_6_31_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0163-4453(79)80007-9"},{"key":"e_1_2_6_32_2","first-page":"5","article-title":"Van \u00e7evresindeki koyunlarda Campylobacter antikorlar\u00fdn\u00fdn ELISA ile saptanmas\u0131","volume":"1","author":"Yard\u0131mc\u0131 H.","year":"1998","journal-title":"Y.Y.\u00dc. Vet. Fak. Derg."},{"key":"e_1_2_6_33_2","first-page":"129","article-title":"Use of ELISA for detection of Campylobacter antibodies in sheep","volume":"18","author":"Yard\u0131mc\u0131 H.","year":"1994","journal-title":"Tr. J. Vet Anim Sci."}],"container-title":["Journal of Veterinary Medicine, Series B"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1046%2Fj.1439-0450.2002.00524.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1046\/j.1439-0450.2002.00524.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,1,3]],"date-time":"2024-01-03T20:36:45Z","timestamp":1704314205000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1046\/j.1439-0450.2002.00524.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2002,4]]},"references-count":32,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2002,4]]}},"alternative-id":["10.1046\/j.1439-0450.2002.00524.x"],"URL":"https:\/\/doi.org\/10.1046\/j.1439-0450.2002.00524.x","archive":["Portico"],"relation":{},"ISSN":["0931-1793"],"issn-type":[{"value":"0931-1793","type":"print"}],"subject":[],"published":{"date-parts":[[2002,4]]}}}