{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T06:47:40Z","timestamp":1760078860329},"reference-count":26,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2002,10,28]],"date-time":"2002-10-28T00:00:00Z","timestamp":1035763200000},"content-version":"vor","delay-in-days":27,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Veterinary Medicine, Series B"],"published-print":{"date-parts":[[2002,10]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>In the present work, evidence of<jats:italic>Listeria monocytogenes<\/jats:italic>antigens based on the avidin\u2013biotin complex (ABC) immunoperoxidase technique was performed on formalin\u2010fixed central nervous system tissues (CNS) from a total of 23 natural cases of encephalitis (four ovine and 19 bovine).<jats:italic>Listeria monocytogenes<\/jats:italic>serotype 4 was isolated from 10 of 17 cultured specimens. Meningoencephalitis characterized by focal necrosis, microabscesses, perivascular cuffing, and gliosis with presence of macrophages and\/or neutrophils was observed at histological examination. Positive<jats:italic>L. monocytogenes<\/jats:italic>antigens were successfully identified by immunohistochemistry (IHC) in the CNS of all 23 cases. Paraffin\u2010embedded tissues assayed were stored up for 17 years. Morbidity of the outbreaks was between 0.3\u20133% and 0.1\u20131% for ovine and bovine cases, respectively. In all the ovine cases, flocks involved were under extensive grazing conditions. In nine of the 19 bovine cases (47.3%), supplementation with corn silage was used. The ABC test can help as a practical tool for the diagnosis of natural cases of<jats:italic>L. monocytogenes<\/jats:italic>encephalitis on formalin\u2010fixed specimens from ovine and bovine.<\/jats:p>","DOI":"10.1046\/j.1439-0450.2002.00586.x","type":"journal-article","created":{"date-parts":[[2003,3,12]],"date-time":"2003-03-12T09:16:09Z","timestamp":1047460569000},"page":"379-383","source":"Crossref","is-referenced-by-count":26,"title":["Demonstration of<i>Listeria monocytogenes<\/i>by Immunohistochemistry in Formalin\u2010Fixed Brain Tissues from Natural Cases of Ovine and Bovine Encephalitis"],"prefix":"10.1111","volume":"49","author":[{"given":"C. M.","family":"Campero","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A. C.","family":"Ode\u00f3n","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A. 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