{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T21:05:13Z","timestamp":1760216713773},"reference-count":46,"publisher":"Wiley","issue":"1-2","license":[{"start":{"date-parts":[[2003,12,10]],"date-time":"2003-12-10T00:00:00Z","timestamp":1071014400000},"content-version":"vor","delay-in-days":1436,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clin Exp Pharma Physio"],"published-print":{"date-parts":[[2000,1,4]]},"abstract":"<jats:title>SUMMARY<\/jats:title><jats:p>1. At present, CO<jats:sub>2<\/jats:sub> is considered to be the most important factor in regulating cerebral blood flow by modification of the interstitial fluid and extracellular pH, but the mechanism by which hypercapnia produces vasodilation is still controversial. In the present paper we investigated the effect of hypercapnia on carbonic anhydrase (CA) activity. We also studied the combined effects of CO<jats:sub>2<\/jats:sub> with either indomethacin or an <jats:sc>L<\/jats:sc>\u2010arginine analogue on CA activity.<\/jats:p><jats:p>2. Nine groups of 12 rabbits each were established. Groups 1\u20134 were ventilated with a mixture of 10% CO<jats:sub>2<\/jats:sub>, 21% O<jats:sub>2<\/jats:sub> and 69% N<jats:sub>2<\/jats:sub> for 20, 60, 120 and 180 min. Group 5 rabbits received 15 mg\/kg bodyweight, i.v., indomethacin and, after 1 h, were ventilated with a mixture of 10% CO<jats:sub>2<\/jats:sub>, 21% O<jats:sub>2<\/jats:sub> and 69% N<jats:sub>2<\/jats:sub> for 2 h. Group 6 animals were ventilated with a mixture of 10% CO<jats:sub>2<\/jats:sub>, 21% O<jats:sub>2<\/jats:sub> and 69% N<jats:sub>2<\/jats:sub> for 2 h and then received indomethacin. Group 7 rabbits received 100 mg\/kg bodyweight, i.v., <jats:italic>N<\/jats:italic><jats:sup>G<\/jats:sup>\u2010monomethyl\u2010<jats:sc>L<\/jats:sc>\u2010arginine (<jats:sc>L<\/jats:sc>\u2010NMMA) and, after 1 h, were ventilated with a mixture of 10% CO<jats:sub>2<\/jats:sub>, 21% O<jats:sub>2<\/jats:sub> and 69% N<jats:sub>2<\/jats:sub> for 2 h. Group 8 rabbits were ventilated for 2 h with a mixture of 10% CO<jats:sub>2<\/jats:sub>, 21% O<jats:sub>2<\/jats:sub> and 69% N<jats:sub>2<\/jats:sub> and were then administered <jats:sc>L<\/jats:sc>\u2010NMMA. Group 9 rabbits received <jats:sc>L<\/jats:sc>\u2010NMMA treatment concomitant with ventilation for 2 h with a mixture of 10% CO<jats:sub>2<\/jats:sub>, 21% O<jats:sub>2<\/jats:sub> and 69% N<jats:sub>2<\/jats:sub>. In all groups, the erythrocyte CA activity was measured, as well as <jats:italic>P<\/jats:italic><jats:sub>a<\/jats:sub><jats:sc>CO<\/jats:sc><jats:sub>2<\/jats:sub> before and after ventilation or treatment.<\/jats:p><jats:p>3. The present study shows that CO<jats:sub>2<\/jats:sub> reduces CA I activity down to complete inhibition and antagonizes the activating effects of indomethacin and <jats:sc>L<\/jats:sc>\u2010NMMA on this isozyme. Our data prove that nitric oxide\u2010 and prostaglandin\u2010induced CA I inhibition is involved in the vasodilation produced by hypercapnia. These results suggest that, due to subsequent pH changes, CA I is directly implicated in the modulation of vascular processes in the organism.<\/jats:p>","DOI":"10.1046\/j.1440-1681.2000.03212.x","type":"journal-article","created":{"date-parts":[[2003,3,11]],"date-time":"2003-03-11T11:06:05Z","timestamp":1047380765000},"page":"95-99","source":"Crossref","is-referenced-by-count":18,"title":["Carbonic Anhydrase I Inhibition By Nitric Oxide: Implications For Mediation Of The Hypercapnia\u2010Induced Vasodilator Response"],"prefix":"10.1111","volume":"27","author":[{"given":"Ioan","family":"Puscas","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marcela","family":"Coltau","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gabriela","family":"Domuta","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael","family":"Baican","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carmen","family":"Puscas","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Radu","family":"Pasca","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2003,12,10]]},"reference":[{"key":"e_1_2_5_2_2","first-page":"16","article-title":"Die Bewegungen des Gehirns und die Veranderungen der Gefassfullung der pia mater.","volume":"69","author":"Donders FC.","year":"1851","journal-title":"Schmidt Fahrbucher"},{"key":"e_1_2_5_3_2","doi-asserted-by":"publisher","DOI":"10.1001\/archneurpsyc.1930.02220120002001"},{"key":"e_1_2_5_4_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI101995"},{"key":"e_1_2_5_5_2","doi-asserted-by":"publisher","DOI":"10.1055\/s-2008-1052397"},{"key":"e_1_2_5_6_2","first-page":"R382","article-title":"Chemical regulation of cerebral blood flow in turtles.","volume":"260","author":"Davies DG.","year":"1991","journal-title":"Am. 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