{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,20]],"date-time":"2026-03-20T11:46:55Z","timestamp":1774007215118,"version":"3.50.1"},"reference-count":25,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2002,1,12]],"date-time":"2002-01-12T00:00:00Z","timestamp":1010793600000},"content-version":"vor","delay-in-days":175,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clin Exp Pharma Physio"],"published-print":{"date-parts":[[2001,7,21]]},"abstract":"<jats:title>SUMMARY<\/jats:title><jats:p>1. Subthreshold concentrations of endothelin (ET)\u20101 enhance the contractile responses to noradrenaline (NA). We investigated possible mechanisms underlying the ET\u20101\u2010induced enhancement of vasoconstrictor responses to NA in rat perfused mesenteric arteries.<\/jats:p><jats:p>2. Perfusion of arteries with subpressor dose of ET\u20101 (3 <jats:bold>\u00d7<\/jats:bold> 10<jats:sup>\u201310<\/jats:sup> mol\/L) significantly potentiated the pressor responses to NA (10<jats:sup>\u20136<\/jats:sup>, 3 <jats:bold>\u00d7<\/jats:bold> 10<jats:sup>\u20136<\/jats:sup> and 10<jats:sup>\u20135<\/jats:sup> mol\/L) and this action of ET\u20101 was endothelium independent.<\/jats:p><jats:p>3. The protein kinase C (PKC) inhibitors staurosporine (10<jats:sup>\u20138<\/jats:sup> mol\/L) and calphostin C (10<jats:sup>\u20137<\/jats:sup> mol\/L) markedly attenuated the ET\u20101\u2010induced enhancement of NA responses. Vasoconstrictor responses to NA were potentiated when vessels were perfused with phorbol 12\u2010myristate 13\u2010acetate (10<jats:sup>\u20138<\/jats:sup> mol\/L).<\/jats:p><jats:p>4. The potentiating effect of ET\u20101 was efficiently suppressed by Y\u201027632 (10<jats:sup>\u20136<\/jats:sup> mol\/L), a selective Rho\u2010kinase inhibitor. In the presence of both staurosporine and Y\u201027632, contractile responses to NA alone were decreased markedly and ET\u20101\u2010induced potentiation was abolished.<\/jats:p><jats:p>5. Both staurosporine and Y\u201027632 decreased contractile responses to NA in arteries of deoxycorticosterone acetate (DOCA)\u2010salt hypertensive rats to levels observed in normotensive control animals.<\/jats:p><jats:p>6. These findings suggest that ET\u20101\u2010mediated potentiation of responses to NA occurs through activation of either PKC or Rho\u2010kinase. This mechanism seems to contribute to the enhanced vasoconstrictor responces to NA observed in DOCA\u2010salt hypertensive rats, in which the responses to NA are enhanced tonically by endogenous vascular ET\u20101.<\/jats:p>","DOI":"10.1046\/j.1440-1681.2001.03484.x","type":"journal-article","created":{"date-parts":[[2003,3,11]],"date-time":"2003-03-11T11:10:27Z","timestamp":1047381027000},"page":"540-544","source":"Crossref","is-referenced-by-count":14,"title":["Mechanisms Of Endothelin\u20101\u2010Induced Potentiation Of Noradrenaline Response In Rat Mesenteric Artery"],"prefix":"10.1111","volume":"28","author":[{"given":"Yasuo","family":"Matsumura","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Satomi","family":"Kita","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tomoko","family":"Okui","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2002,1,12]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.HYP.21.6.752"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1254\/jjp.72.261"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.HYP.33.2.759"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.physiol.61.1.391"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/0024-3205(91)90249-B"},{"key":"e_1_2_6_7_2","doi-asserted-by":"crossref","unstructured":"TakaokaM KuroT MatsumuraY.Role of endothelin in the pathogenesis of acute renal failure.Drug News Perspect.2000;13: 141\u20136.","DOI":"10.1358\/dnp.2000.13.3.858437"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.HYP.22.1.78"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.82.1.188"},{"key":"e_1_2_6_10_2","doi-asserted-by":"crossref","unstructured":"Wong\u2010DustingHK LaM RandMJ.Effect of endothelin\u20101 on responses of isolated blood vessels to vasoconstrictor agents.J. 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