{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T14:26:49Z","timestamp":1790260009468,"version":"4.1.0"},"reference-count":40,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2003,8,11]],"date-time":"2003-08-11T00:00:00Z","timestamp":1060560000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Gastro and Hepatol"],"published-print":{"date-parts":[[2003,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:bold>Background and Aims:\u2002<\/jats:bold> Genetic mechanisms involved in the development of gastric B\u2010cell lymphomas remain unclear. The aim of the present study was to clarify the roles of mutations of the <jats:italic>p53<\/jats:italic> and K\u2010<jats:italic>ras<\/jats:italic> genes, and microsatellite instability (MSI) in the development of gastric B\u2010cell lymphomas.<\/jats:p><jats:p><jats:bold>Methods:\u2002<\/jats:bold> We investigated p53 immunoreactivity, mutations of the K\u2010<jats:italic>ras<\/jats:italic> gene, and MSI in 27 gastric marginal zone B\u2010cell lymphomas of mucosa\u2010associated lymphoid tissue type (MZBCL) and 24 diffuse large B\u2010cell lymphomas (DLBCL). p53 immunoreactivity was examined using a monoclonal antibody, DO\u20107. Mutation of the K\u2010<jats:italic>ras<\/jats:italic> gene was detected by polymerase chain reaction\u2010single strand conformation polymorphism (PCR\u2010SSCP) analysis. MSI was examined at five microsatellite loci with a microsatellite assay. Cases were classified as having high\u2010frequency MSI (MSI\u2010H) (\u2265\u20032 loci showing instability), low\u2010frequency MSI (MSI\u2010L) (only one locus showing instability), or as microsatellite stable.<\/jats:p><jats:p><jats:bold>Results:\u2002<\/jats:bold> p53 immunoreactivity was detected in 1 of 16 (6%) MZBCL and 8 of 19 (42%) DLBCL. Frequency of p53 immunoreactivity in DLBCL was significantly higher than that in MZBCL (<jats:italic>P<\/jats:italic>\u2003=\u20030.018). MSI\u2010H was detected only in 1 of 20 (5%) DLBCL. None of the cases examined showed mutation of the K\u2010<jats:italic>ras<\/jats:italic> gene.<\/jats:p><jats:p><jats:bold>Conclusions:\u2002<\/jats:bold> These data suggest that mutations of the <jats:italic>p53<\/jats:italic> gene may play an important role in the development of gastric DLBCL, and that mutations of the K\u2010<jats:italic>ras<\/jats:italic> gene and MSI may be involved in little part of the development of gastric B\u2010cell lymphomas.<\/jats:p>","DOI":"10.1046\/j.1440-1746.2003.03120.x","type":"journal-article","created":{"date-parts":[[2003,8,11]],"date-time":"2003-08-11T09:06:15Z","timestamp":1060592775000},"page":"1047-1053","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["p53 expression, K\u2010<i>ras<\/i> gene mutation and microsatellite instability in gastric B\u2010cell lymphomas"],"prefix":"10.1111","volume":"18","author":[{"given":"TORU","family":"HIYAMA","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"SHINJI","family":"TANAKA","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"HIROSHI","family":"MASUDA","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"HIDEYUKI","family":"SHIMA","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"KAZUHIRO","family":"KOSE","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"HANDAN","family":"TUNCEL","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"MASANORI","family":"ITO","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"YASUHIKO","family":"KITADAI","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"MASAHARU","family":"SUMII","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"NAOMI","family":"UEMURA","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"MASAHARU","family":"YOSHIHARA","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"FUMIO","family":"SHIMAMOTO","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"KEN","family":"HARUMA","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"KAZUAKI","family":"CHAYAMA","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2003,8,11]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00735227"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0142(19831015)52:8<1410::AID-CNCR2820520813>3.0.CO;2-3"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.annonc.a059177"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V84.5.1361.1361"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1440-1746.2001.02519.x"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/0046-8177(94)90060-4"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2559.1997.5450786.x"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9440(10)64628-7"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.1999.17.12.3835"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9440(10)65611-8"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1053\/gast.1997.v112.pm9178679"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-9896(199704)181:4<381::AID-PATH787>3.0.CO;2-I"},{"key":"e_1_2_6_14_2","first-page":"289","article-title":"c\u2010myc gene mutation in gastric mucosa\u2010associated lymphoid tissue (MALT) lymphoma and diffuse large B\u2010cell lymphoma","volume":"8","author":"Hiyama T","year":"2001","journal-title":"Oncol. 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