{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,12]],"date-time":"2026-05-12T22:53:16Z","timestamp":1778626396127,"version":"3.51.4"},"reference-count":36,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2001,12,25]],"date-time":"2001-12-25T00:00:00Z","timestamp":1009238400000},"content-version":"vor","delay-in-days":572,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Neuropathology"],"published-print":{"date-parts":[[2000,6]]},"abstract":"<jats:p>Macrophage\u2010colony\u2010stimulating factor (M\u2010CSF) regulates the survival, proliferation and differentiation of the mononuclear phagocyte lineage. Osteopetrotic (<jats:italic>op\/op<\/jats:italic>) mice defective in producing functional M\u2010CSF were used in order to investigate the role of M\u2010CSF on the development of microglia and brain macrophages and the expression of scavenger receptor (SR). Adult <jats:italic>op\/op<\/jats:italic> and littermate mice at 10\u201347 weeks of age were investigated by immunohistochemistry with a panel of monoclonal antibodies (F4\/80, Mac\u20101, anti\u2010major histocompatibility complex (MHC) class II and anti\u2010SR), electron microscopy and reverse transcriptase\u2013polymerase chain reaction (RT\u2010PCR). Microglia were weakly immunolabeled with F4\/80 and Mac\u20101 in <jats:italic>op\/op<\/jats:italic> and littermate mice, but the number of microglia in <jats:italic>op\/op<\/jats:italic> mice was reduced in the cerebrum, cerebellum and brainstem compared with that of normal littermates. The numbers of Mac\u20101\u2010positive microglia in <jats:italic>op\/op<\/jats:italic> mice was 39% (pons) and 30% (cerebellar cortex) lower than that in normal littermates (<jats:italic>P<\/jats:italic><jats:bold>&lt;<\/jats:bold> 0.05). In addition, the microglia cell processes in <jats:italic>op\/op<\/jats:italic> mice were often shorter than those in control mice. In <jats:italic>op\/op<\/jats:italic> and littermate mice, both MHC class II and SR were present in perivascular cells and macrophages of the leptomeninx and choroid plexus. Ultrastructurally, perivascular cells appeared to be immature, since their cytoplasm was narrow and contained few inclusion bodies compared with those of control mice. Reverse transcriptase\u2013polymerase chain reaction showed a weak expression for SR mRNA in the brains of <jats:italic>op\/op<\/jats:italic> mice as well as littermate mice. These results indicate that microglia are partly dependent on M\u2010CSF for their proliferation and differentiation and that M\u2010CSF has no significant effect on the expression of SR in the physiological brain. The study also suggests that M\u2010CSF affects the maturation of perivascular cells at the ultrastructural level.<\/jats:p>","DOI":"10.1046\/j.1440-1789.2000.00286.x","type":"journal-article","created":{"date-parts":[[2003,3,12]],"date-time":"2003-03-12T15:06:03Z","timestamp":1047481563000},"page":"134-142","source":"Crossref","is-referenced-by-count":37,"title":["Effects of macrophage\u2010colony\u2010stimulating factor deficiency on the maturation of microglia and brain macrophages and on their expression of scavenger 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