{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,11]],"date-time":"2026-02-11T15:12:07Z","timestamp":1770822727983,"version":"3.50.1"},"reference-count":15,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2001,12,25]],"date-time":"2001-12-25T00:00:00Z","timestamp":1009238400000},"content-version":"vor","delay-in-days":693,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathology International"],"published-print":{"date-parts":[[2000,2]]},"abstract":"<jats:p>To examine whether or not optically clear nuclei are one of the fixation artifacts in papillary carcinoma of the thyroid. Experiments using an optimal immersion fixation method by making thin\u2010sliced specimens, 5 <jats:bold>\u00d7<\/jats:bold> 2 <jats:bold>\u00d7<\/jats:bold> 2 mm in size were adopted. Fixatives tested were 10% formalin, 10% buffered formalin, mixture of alcohol and formalin (4 : 1), 95% alcohol, 4% phosphate\u2010buffered paraformaldehyde, Carnoy\u2019s fixative, Bouin\u2019s fixative and various concentrations of formalin. Tested thyroid lesions in total were: cases with papillary carcinoma (38); follicular carcinoma (one); follicular adenoma (15); adenomatous goiter (11); Hashimoto\u2019s thyroiditis (two); Grave\u2019s disease (five); and normal thyroid tissue around tumor (five). Routinely processed papillary carcinoma showed a high occurrence (64.6%) of the optically clear nuclei contrasted by a low occurrence (almost 0%) in other lesions. However, this rate in papillary carcinoma decreased (<jats:bold>&lt;<\/jats:bold> 35.3%) in all experimental groups irrespective of the choice of fixatives. Frequent occurrence (61.9\u201366.6%) of the optically clear nuclei was reproduced in papillary carcinoma fixed in a higher concentration of formalin (40\u201360%) in the experimental groups. These results demonstrated that the optically clear nuclei are one of the fixation artifacts, although still useful as a diagnostic criterion.<\/jats:p>","DOI":"10.1046\/j.1440-1827.2000.01013.x","type":"journal-article","created":{"date-parts":[[2003,3,12]],"date-time":"2003-03-12T17:14:32Z","timestamp":1047489272000},"page":"113-118","source":"Crossref","is-referenced-by-count":14,"title":["Optically clear nuclei in papillary carcinoma of the thyroid: Demonstration of one of the fixation artifacts and its practical usefulness"],"prefix":"10.1111","volume":"50","author":[{"given":"Hiroshi","family":"Naganuma","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Harunobu","family":"Murayama","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Noriko","family":"Ohtani","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kiyoshi","family":"Takaya","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoko","family":"Mori","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nobumitsu","family":"Sakai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kenichi","family":"Kakudo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2001,12,25]]},"reference":[{"key":"e_1_2_6_2_2","volume-title":"Carcinoma of the Thyroid Gland. 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