{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T06:25:03Z","timestamp":1789971903306,"version":"4.0.1"},"reference-count":30,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2001,12,21]],"date-time":"2001-12-21T00:00:00Z","timestamp":1008892800000},"content-version":"vor","delay-in-days":264,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathology International"],"published-print":{"date-parts":[[2001,4]]},"abstract":"<jats:p>To investigate the molecular mechanism for enhanced fibrous stroma formation in intrahepatic cholangiocarcinoma (ICC), we surveyed the expression pattern of basement\u2010membrane\u2010type heparan sulfate proteoglycan (HSPG; also known as perlecan) at the core protein and the mRNA level in ICC as well as in other liver neoplasms and reactive hepatic diseases. Immunohistochemistry of paraffin\u2010embedded liver sections with hyaluronidase pretreatment showed that HSPG was present in small amounts in normal liver around the bile ducts and the blood vessels within the portal area. There was no evident expression within the hepatic lobules. Intense immunoexpression of HSPG was seen in the tumor\u2010specific fibro\u2010myxoid stroma of ICC and metastatic liver cancer originating from the colon. However, tumor\u2010specific stroma of hepatocellular carcinomas showed little or no expression of HSPG. At the mRNA level, signals for HSPG were found in tumor cells of cholangiocarcinoma and metastatic colonic carcinomas, and in myofibroblasts in the tumor fibro\u2010myxoid\u2010specific stroma. From immunoprecipitation and reverse transcription\u2013polymerase chain reaction (RT\u2013PCR) analyses, a cultured human intrahepatic cholangiocarcinoma cell line (CCKS1), was found to express high levels of HSPG core protein and mRNA. These findings suggest that biliary and metastatic colon carcinoma cells as well as stromal myofibroblasts have a potential for HSPG production. In order to investigate the growth, invasion and metastatic ability of ICC, further study of the \u2018self\u2010made\u2019 stromal component of ICC may provide a new approach.<\/jats:p>","DOI":"10.1046\/j.1440-1827.2001.01201.x","type":"journal-article","created":{"date-parts":[[2003,3,12]],"date-time":"2003-03-12T17:16:43Z","timestamp":1047489403000},"page":"248-256","source":"Crossref","is-referenced-by-count":37,"title":["Enhanced expression of basement\u2010membrane\u2010type heparan sulfate proteoglycan in tumor fibro\u2010myxoid stroma of intrahepatic cholangiocarcinoma"],"prefix":"10.1111","volume":"51","author":[{"given":"Hemragul","family":"Sabit","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Koichi","family":"Tsuneyama","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tomonori","family":"Shimonishi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kenichi","family":"Harada","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun","family":"Cheng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroko","family":"Ida","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takashi","family":"Saku","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katsuhiko","family":"Saito","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yasuni","family":"Nakanuma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2001,12,21]]},"reference":[{"key":"e_1_2_5_2_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI3793"},{"key":"e_1_2_5_3_2","doi-asserted-by":"publisher","DOI":"10.1002\/hep.510240310"},{"key":"e_1_2_5_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/(sici)1097-0215(19970620)74:3<335::aid-ijc18>3.3.co;2-4"},{"key":"e_1_2_5_5_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00192112"},{"key":"e_1_2_5_6_2","doi-asserted-by":"publisher","DOI":"10.1097\/00006676-199411000-00015"},{"key":"e_1_2_5_7_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI119377"},{"key":"e_1_2_5_8_2","doi-asserted-by":"publisher","DOI":"10.1002\/jnr.490380610"},{"key":"e_1_2_5_9_2","doi-asserted-by":"publisher","DOI":"10.1006\/jsre.1995.1008"},{"key":"e_1_2_5_10_2","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.2910480119"},{"key":"e_1_2_5_11_2","doi-asserted-by":"crossref","first-page":"11 216","DOI":"10.1016\/S0021-9258(19)78113-7","article-title":"Interaction of hepatocyte growth factor with heparan sulfate. 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