{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,11]],"date-time":"2026-02-11T15:31:55Z","timestamp":1770823915598,"version":"3.50.1"},"reference-count":16,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2001,12,21]],"date-time":"2001-12-21T00:00:00Z","timestamp":1008892800000},"content-version":"vor","delay-in-days":142,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathology International"],"published-print":{"date-parts":[[2001,8]]},"abstract":"<jats:p>Evaluation of <jats:italic>HER\u20102\/neu<\/jats:italic> status is important in the management of patients with breast carcinoma, especially in determining the possible application of trastuzumab, a humanized anti\u2010HER\u20102\/neu monoclonal antibody. Chromogenic <jats:italic>in situ<\/jats:italic> hybridization (CISH) detection of the <jats:italic>HER\u20102\/neu<\/jats:italic> oncogene is a newly developed <jats:italic>in situ<\/jats:italic> hybridization method that utilizes a robust and unique\u2010sequence DNA probe labeled with digoxygenin, and sequential incubations with antidigoxygenin fluorescein, antifluorescein peroxidase, and diaminobenzidine. In this study, we examined 20 archival specimens of human breast carcinoma using CISH, and we correlated findings with immunohistochemical findings for HER\u20102\/neu. HER\u20102\/neu immunohistochemistry was carried out with HercepTest<jats:sup>TM<\/jats:sup>, a standardized immunohistochemical examination system for <jats:italic>HER\u20102\/neu<\/jats:italic> overexpression in surgical pathology specimens. CISH analysis could be done in 18 out of 20 cases examined. Gene copy signals for <jats:italic>HER\u20102\/neu<\/jats:italic> were recognized as intranuclear brown dots in both neoplastic and non\u2010neoplastic cells. Seven carcinomas showed an increased number or size of signals and were interpreted as being positive for <jats:italic>HER\u20102\/neu<\/jats:italic> amplification. Eight cases were positive with the HercepTest<jats:sup>TM<\/jats:sup>. Seven out of eight carcinoma cases found to overexpress immunoreactive HER\u20102\/neu also demonstrated <jats:italic>HER\u20102\/neu<\/jats:italic> gene amplification following CISH analysis. There was a significant correlation between immunohistochemical and CISH analyses (<jats:italic>P<\/jats:italic><jats:bold>&lt;<\/jats:bold> 0.001). We found that CISH was a specific, sensitive and easily applicable method for the detection of <jats:italic>HER\u20102\/neu<\/jats:italic> gene amplification, which may be used together with immunohistochemical examination for the evaluation of patients with breast carcinoma.<\/jats:p>","DOI":"10.1046\/j.1440-1827.2001.01255.x","type":"journal-article","created":{"date-parts":[[2003,3,12]],"date-time":"2003-03-12T17:17:27Z","timestamp":1047489447000},"page":"579-584","source":"Crossref","is-referenced-by-count":45,"title":["Chromogenic <i>in situ<\/i> hybridization analysis of <i>HER\u20102\/neu<\/i> status in breast carcinoma: Application in screening of patients for trastuzumab (Herceptin<sup>\u00ae<\/sup>) therapy"],"prefix":"10.1111","volume":"51","author":[{"given":"Hiroyuki","family":"Kumamoto","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hironobu","family":"Sasano","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takahiro","family":"Taniguchi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takashi","family":"Suzuki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takuya","family":"Moriya","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ryo","family":"Ichinohasama","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2001,12,21]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/290261a0"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.2992090"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/0304-419X(94)90012-4"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.3012781"},{"key":"e_1_2_6_6_2","first-page":"S53","article-title":"Her\u20102\/neu (c\u2010erb\u2010B2) gene and protein in breast cancer.","volume":"112","author":"Ross JS","year":"1999","journal-title":"Am. J. Clin. Pathol."},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.3798106"},{"key":"e_1_2_6_8_2","first-page":"1238","article-title":"Correlation of c\u2010erbB\u20102 gene amplification and protein expression in human breast carcinoma with nodal status and nuclear grading.","volume":"48","author":"Berger MS","year":"1988","journal-title":"Cancer Res."},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.89.10.4285"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.1999.17.9.2639"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.1999.17.7.1983"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9440(10)64785-2"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1440-1827.2001.01163.x"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(87)92736-X"},{"key":"e_1_2_6_15_2","first-page":"1247","article-title":"The combination of in situ hybridization and immunohistochemical analysis: an evaluation of Her\u20102\/neu expression in paraffin\u2010embedded breast carcinomas and adjacent normal\u2010appearing breast epithelium.","volume":"10","author":"Ratcliffe N","year":"1997","journal-title":"Mod. Pathol."},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1038\/modpathol.3880007"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9440(10)65727-6"}],"container-title":["Pathology International"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1046%2Fj.1440-1827.2001.01255.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1046\/j.1440-1827.2001.01255.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,17]],"date-time":"2023-10-17T01:27:37Z","timestamp":1697506057000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1046\/j.1440-1827.2001.01255.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2001,8]]},"references-count":16,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2001,8]]}},"alternative-id":["10.1046\/j.1440-1827.2001.01255.x"],"URL":"https:\/\/doi.org\/10.1046\/j.1440-1827.2001.01255.x","archive":["Portico"],"relation":{},"ISSN":["1320-5463","1440-1827"],"issn-type":[{"value":"1320-5463","type":"print"},{"value":"1440-1827","type":"electronic"}],"subject":[],"published":{"date-parts":[[2001,8]]}}}