{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T18:28:46Z","timestamp":1761762526198},"reference-count":30,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2002,3,1]],"date-time":"2002-03-01T00:00:00Z","timestamp":1014940800000},"content-version":"vor","delay-in-days":151,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathology International"],"published-print":{"date-parts":[[2001,10]]},"abstract":"<jats:p>To investigate the effectiveness of chromogen <jats:italic>in situ<\/jats:italic> hybridization (CISH) in the diagnosis of breast tumors, numerical alterations of chromosome 1 were examined by CISH and fluorescence <jats:italic>in situ<\/jats:italic> hybridization (FISH) methods, and the presence of der(16)t(1;16) was also examined by FISH in imprinted cytology specimens from resected tissues of 14 carcinomas and five non\u2010malignant lesions. The modal signal counts of chromosome 1 were compared between the specimens processed by CISH and FISH for each case. Aneusomies of the long arm of chromosome 1 were detected in 10 (71%) carcinomas as the major clones by both methods. In addition, one atypical papilloma demonstrated tetrasomy of 1q12 as a major clone by CISH, but such a clone was at first overlooked by FISH. Four other benign lesions showed disomic 1q12 signals as a major clone by both CISH and FISH. As additional information from FISH, eight cancers showed structural or numerical alterations of chromosome 16, and four showed der(16)t(1;16). In total, 10 carcinomas showed chromosome 16 alterations, and all of these overlapped with the carcinomas with 1q12 aneusomies. The CISH method provided almost the same results as the FISH method, and both methods were considered applicable in supportive diagnosis of cytological specimens of breast tumors. In addition, the CISH method was superior in the detection of numerical alterations in carcinoma cells by referring to the morphology of cells and in the detection of significant clones which might be missed under dark\u2010field microscopy.<\/jats:p>","DOI":"10.1046\/j.1440-1827.2001.01282.x","type":"journal-article","created":{"date-parts":[[2003,3,12]],"date-time":"2003-03-12T17:15:26Z","timestamp":1047489326000},"page":"786-791","source":"Crossref","is-referenced-by-count":4,"title":["Detection of numerical alterations of chromosome 1 in cytopathological specimens of breast tumors by chromogen <i>in situ<\/i> hybridization"],"prefix":"10.1111","volume":"51","author":[{"given":"Teruko","family":"Takarabe","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hitoshi","family":"Tsuda","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Satoshi","family":"Okada","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takashi","family":"Fukutomi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Setsuo","family":"Hirohashi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2002,3]]},"reference":[{"key":"e_1_2_6_2_2","first-page":"7184","article-title":"Allelotype of breast cancer: Cumulative allele losses promote tumor progression in primary breast cancer.","volume":"50","author":"Sato T","year":"1990","journal-title":"Cancer Res."},{"key":"e_1_2_6_3_2","first-page":"1990","article-title":"Two distinct regions involved in 1p deletion in human primary breast cancer.","volume":"53","author":"Bi\u00e8che I","year":"1993","journal-title":"Cancer Res."},{"key":"e_1_2_6_4_2","first-page":"4486","article-title":"Refinement of regional loss of heterozygosity for chromosome 11p15.5 in human breast tumors.","volume":"53","author":"Winqvist R","year":"1993","journal-title":"Cancer Res."},{"key":"e_1_2_6_5_2","first-page":"3021","article-title":"Deletion of two separate regions on chromosome 3p in breast cancers.","volume":"54","author":"Chen LC","year":"1994","journal-title":"Cancer Res."},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1002\/gcc.2870040203"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/0165-4608(90)90143-X"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1002\/gcc.2870050310"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1002\/gcc.2870120304"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1159\/000130716"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/0165-4608(81)90066-2"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/0165-4608(86)90368-7"},{"key":"e_1_2_6_13_2","first-page":"123","article-title":"Loss and gain of distinct regions of chromosome 1q in primary breast cancer.","volume":"1","author":"Bi\u00e8che I","year":"1995","journal-title":"Clin. 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