{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T03:11:00Z","timestamp":1761707460514},"reference-count":46,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2002,4,5]],"date-time":"2002-04-05T00:00:00Z","timestamp":1017964800000},"content-version":"vor","delay-in-days":63,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathology International"],"published-print":{"date-parts":[[2002,2]]},"abstract":"<jats:p> <jats:italic>Helicobacter pylori<\/jats:italic> infection is currently considered to be a major cause of acute and chronic gastritis, and of gastric and duodenal ulcers. Superoxide dismutase (SOD) is well known for scavenging superoxide radicals such as reactive oxygen species (ROS), subsequently protecting cells from oxidative injury, and for maintaining tissue homeostasis. In this study, we therefore evaluated the level of SOD activity and protein expression, as well as various factors associated with oxidative injury, in <jats:italic>H. pylori\u2010<\/jats:italic>positive (<jats:italic>n<\/jats:italic>\u2003=\u200346) and \u2010negative (<jats:italic>n<\/jats:italic>\u2003=\u200328) gastric mucosa obtained from endoscopy, in order to elucidate the possible biological significance of SOD in these mucosa. Overall SOD activity was significantly higher in <jats:italic>H. pylori<\/jats:italic>\u2010positive mucosa (15.5\u2003\u00b1 7.0\u2003U\/mg protein) than in negative mucosa (9.2\u2003\u00b1\u200310.6\u2003U\/mg protein), and decreased markedly following <jats:italic>H. pylori<\/jats:italic> eradication (8.2\u2003\u00b1\u20034.2\u2003U\/mg protein). Enzyme\u2010linked immunosorbent assay (ELISA) analysis of SOD revealed that the manganese SOD (Mn\u2010SOD) level in <jats:italic>H. pylori<\/jats:italic>\u2010positive mucosa (1166.7\u2003\u00b1\u2003435.2\u2003ng\/mg protein) was significantly higher than in control tissues (446.3\u2003\u00b1\u2003435.3\u2003ng\/mg protein) and in mucosa obtained following eradication therapy (431.9\u2003\u00b1\u2003189.9\u2003ng\/mg protein). The level of Mn\u2010SOD protein showed a significant correlation with degree of inflammation in the gastric mucosa. Moreover, Mn\u2010SOD immunolocalization patterns were well correlated with the activity and protein levels evaluated by ELISA. Factors presumably associated with oxidative injury in human gastric mucosa, including terminal deoxynucleotidyl transferase\u2010mediated dUTP\u2010biotin nick\u2010end labeling, Ki\u201067, 8\u2010hydroxydeoxyguanosine and single\u2010stranded DNA, were all significantly higher in <jats:italic>H. pylori<\/jats:italic>\u2010positive gastric mucosa than in control tissue and in tissue following eradication. These results all suggest that Mn\u2010SOD, but not cytoplasmic copper\u2013zinc SOD, plays an important role as an anti\u2010oxidant against ROS generated in <jats:italic>H. pylori<\/jats:italic>\u2010infected gastric mucosa and, sub\u00adsequently, in the maintenance of cell turnover in gastric mucosa.<\/jats:p>","DOI":"10.1046\/j.1440-1827.2002.01331.x","type":"journal-article","created":{"date-parts":[[2003,3,12]],"date-time":"2003-03-12T17:18:23Z","timestamp":1047489503000},"page":"110-118","source":"Crossref","is-referenced-by-count":21,"title":["Analysis of cell damage in <i>Helicobacter pylori<\/i>\u2010associated gastritis"],"prefix":"10.1111","volume":"52","author":[{"given":"Kenji","family":"Noguchi","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katsuaki","family":"Kato","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takuya","family":"Moriya","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takashi","family":"Suzuki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michiya","family":"Saito","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tohru","family":"Kikuchi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jing","family":"Yang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Akira","family":"Imatani","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hitoshi","family":"Sekine","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Syuichi","family":"Ohara","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takayoshi","family":"Toyota","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tooru","family":"Shimosegawa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hironobu","family":"Sasano","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2002,4,5]]},"reference":[{"key":"e_1_2_5_2_2","doi-asserted-by":"publisher","DOI":"10.1001\/jama.272.1.65"},{"key":"e_1_2_5_3_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199110173251603"},{"key":"e_1_2_5_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/0140-6736(93)90938-D"},{"key":"e_1_2_5_5_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa001999"},{"key":"e_1_2_5_6_2","first-page":"229","article-title":"Differences in Helicobacter pylori associated gastritis in the antrum and body of the stomach","volume":"28","author":"Stolte M","year":"1990","journal-title":"Z. 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