{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,17]],"date-time":"2023-10-17T05:25:42Z","timestamp":1697520342378},"reference-count":45,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2008,9,5]],"date-time":"2008-09-05T00:00:00Z","timestamp":1220572800000},"content-version":"vor","delay-in-days":2105,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathology International"],"published-print":{"date-parts":[[2002,12]]},"abstract":"<jats:p>It is often difficult to differentiate extranodal marginal zone B\u2010cell lymphoma of mucosa\u2010associated lymphoid tissue (MALT lymphoma) from non\u2010neoplastic inflammatory conditions. Demonstration of clonal lymphoid proliferation by molecular procedures is important for accurate diagnosis. We examined the clonal population of B\u2010cell lymphomas in nine cases of thyroid and two cases of salivary gland B\u2010cell lymphoma using semi\u2010nested polymerase chain reaction (PCR)\u2010based assay for IgH gene arrangement and reverse transcription (RT)\u2010PCR single\u2010strand conformation polymorphism (SSCP) for the detection of IgL gene rearrangement. Clonality was evident in nine out of 11 cases of B\u2010cell lymphomas examined by PCR, and in six of eight cases by RT\u2010PCR SSCP. In addition, analysis of VH families was performed in eight cases. Although VH3 family was frequently used, each case demonstrated the VH4, VH5 or VH6 family. It is possible that the normal counterpart of thyroid or salivary gland lymphoma might be different from peripheral blood B lymphocytes, which usually use VH3 family. Our results indicate that although no clonality was noted in one case by both PCR and SSCP, these molecular methods are useful as supplementary diagnostic tests for both thyroid and salivary gland lymphomas.<\/jats:p>","DOI":"10.1046\/j.1440-1827.2002.01418.x","type":"journal-article","created":{"date-parts":[[2003,3,12]],"date-time":"2003-03-12T17:18:18Z","timestamp":1047489498000},"page":"747-754","source":"Crossref","is-referenced-by-count":2,"title":["Rearrangement of immunoglobulin heavy and light chains and VH family in thyroid and salivary gland lymphomas"],"prefix":"10.1111","volume":"52","author":[{"given":"Kei","family":"Kato","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Koichi","family":"Ohshima","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Satoshi","family":"Shiokawa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takao","family":"Shibata","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Junji","family":"Suzumiya","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masahiro","family":"Kikuchi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2008,9,5]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2559.1978.tb01710.x"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0046-8177(88)80287-9"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2559.1996.268311.x"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.3109\/03009749509095178"},{"key":"e_1_2_6_6_2","first-page":"337","article-title":"Non\u2010Hodgkin's lymphoma associated with primary Sj\u00f6gren's syndrome","volume":"1","author":"Pariente D","year":"1992","journal-title":"Eur. 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