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Then, specific oligodeoxynucleotides were synthesized for in situ hybridization experiments. Both \u03b14 and \u03b22 mRNA signals were widely distributed in the brain, being stronger in the thalamus and in the dopaminergic cells of the mesencephalon. Most brain nuclei displayed both \u03b14 and \u03b22 signals with the exception of some basal ganglia regions and the reticular thalamic nucleus which were devoid of \u03b14 signal. \u03b16 and \u03b23 mRNA signals were selectively concentrated in the substantia nigra and the medial habenula. The strongest signals for \u03b13 or \u03b24 mRNAs were found in the epithalamus (medial habenula and pineal gland), whereas there were no specific \u03b13 or \u03b24 signals in mesencephalic dopaminergic nuclei. \u03b15 and \u03b17 mRNA signals were found in several brain areas, including cerebral cortex, thalamus and substantia nigra, although at a lower level than \u03b14 and \u03b22. The distribution of \u03b13, \u03b14, \u03b15, \u03b16, \u03b17, \u03b22, \u03b23 and \u03b24 subunit mRNAs in the monkey is substantially similar to that observed in rodent brain. Surprisingly, \u03b12 mRNA signal was largely distributed in the Macaca brain, at levels comparable with those of \u03b14 and \u03b22. This observation represents the main difference between rodent and Macaca subunit mRNA distribution and suggests that, besides \u03b14\u03b22*, \u03b12\u03b22* nAChRs constitute a main nAChR isoform in primate brain.<\/jats:p>","DOI":"10.1046\/j.1460-9568.2000.00262.x","type":"journal-article","created":{"date-parts":[[2003,3,6]],"date-time":"2003-03-06T14:27:57Z","timestamp":1046960877000},"page":"3664-3674","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":142,"title":["Localization of nAChR subunit mRNAs in the brain of Macaca mulatta"],"prefix":"10.1111","volume":"12","author":[{"given":"Zhi\u2010Yan","family":"Han","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicolas","family":"Le Nov\u00e8re","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michele","family":"Zoli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"suffix":"Jr","given":"Joseph A.","family":"Hill","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicolas","family":"Champtiaux","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean\u2010Pierre","family":"Changeux","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2001,12,24]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"crossref","first-page":"6475","DOI":"10.1523\/JNEUROSCI.19-15-06475.1999","article-title":"Ultrastructural localization of the \u03b14\u2010subunit of the neuronal acetylcholine nicotinic receptor in the rat substanti nigra","volume":"19","author":"Arroyo\u2010Jim\u00e9nez M.M.","year":"1999","journal-title":"J. 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