{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T16:26:16Z","timestamp":1789230376706,"version":"build-2803163510"},"reference-count":26,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2008,10,9]],"date-time":"2008-10-09T00:00:00Z","timestamp":1223510400000},"content-version":"vor","delay-in-days":2899,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Eur J of Neuroscience"],"published-print":{"date-parts":[[2000,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>In order to explore the mechanisms by which \u03b1\u2010latrotoxin activates neurotransmitter release, we have characterized its effects by patch\u2010clamp methods on cells heterologously expressing its receptors, latrophilin\u20101 or neurexin\u2010I\u03b1. Application of \u03b1\u2010latrotoxin (1\u2003n <jats:sc>m<\/jats:sc>) to cells expressing rat latrophilin or neurexin, but not mock\u2010transfected cells, induced a cationic conductance. In cells expressing latrophilin, current development was slow in the absence of divalent cations, but was accelerated by Ca<jats:sup>2+<\/jats:sup> or Mg<jats:sup>2+<\/jats:sup>. In cells expressing neurexin, \u03b1\u2010latrotoxin did not elicit currents in the absence of Ca<jats:sup>2+<\/jats:sup>. The toxin\u2010induced conductance was rectifying, persistent, permeable to monovalent and divalent cations, but blocked by La<jats:sup>3+<\/jats:sup>. Single\u2010channel recording revealed a permanently open state, with the same unitary conductance irrespective of whether cells expressed latrophilin or neurexin. Therefore, while pore formation displayed differences consistent with the reported properties of \u03b1\u2010latrotoxin binding to latrophilin and neurexin, the pores induced by \u03b1\u2010latrotoxin had identical properties. These results suggest that after anchoring to either of its nerve terminal receptors, \u03b1\u2010latrotoxin inserts into the membrane and constitutes a single type of transmembrane ion pore.<\/jats:p>","DOI":"10.1046\/j.1460-9568.2000.00282.x","type":"journal-article","created":{"date-parts":[[2003,3,6]],"date-time":"2003-03-06T14:28:18Z","timestamp":1046960898000},"page":"3953-3962","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":36,"title":["\u03b1\u2010Latrotoxin forms calcium\u2010permeable membrane pores via interactions with latrophilin or neurexin"],"prefix":"10.1111","volume":"12","author":[{"given":"Catherine","family":"Van Renterghem","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C\u00e9cile","family":"Iborra","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicole","family":"Martin\u2010Moutot","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vera","family":"Lelianova","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuri","family":"Ushkaryov","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Seagar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2008,10,9]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.18-08-02914.1998"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1152\/jn.1996.76.5.3149"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.270.41.23903"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/17.14.3909"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.271.38.23239"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.948756"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00211072"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/17.21.6188"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2736(92)90262-K"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(00)80332-3"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/17.3.648"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.272.34.21504"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.18-16-06113.1998"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/0306-4522(83)90238-5"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7793.1997.481bj.x"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1471-4159.1998.71041339.x"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/71247"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01869630"},{"key":"e_1_2_6_20_1","first-page":"917","article-title":"Mode of action of alpha\u2010latrotoxin: role of divalent cations in Ca2 (+) \u2010dependent and Ca2 (+) \u2010independent effects mediated by the toxin","volume":"38","author":"Rosenthal L.","year":"1990","journal-title":"Mol. 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