{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T02:25:08Z","timestamp":1791080708349,"version":"4.1.0"},"reference-count":33,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2008,10,9]],"date-time":"2008-10-09T00:00:00Z","timestamp":1223510400000},"content-version":"vor","delay-in-days":2899,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Eur J of Neuroscience"],"published-print":{"date-parts":[[2000,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Adult dorsal root ganglia (DRG) have been shown to express a wide range of voltage\u2010gated sodium channel \u03b1\u2010subunits. However, of the auxiliary subunits, \u03b21 is expressed preferentially in only large\u2010 and medium\u2010diameter neurons of the DRG while \u03b22 is absent in all DRG cells. In view of this, we have compared the distribution of \u03b21 in rat DRG and spinal cord with a novel, recently cloned \u03b21\u2010like subunit, \u03b23. In situ hybridization studies demonstrated high levels of \u03b23 mRNA in small\u2010diameter c\u2010fibres, while \u03b21 mRNA was virtually absent in these cell types but was expressed in 100% of large\u2010diameter neurons. In the spinal cord, \u03b23 transcript was present specifically in layers I\/II (substantia gelatinosa) and layer X, while \u03b21 mRNA was expressed in all laminae throughout the grey matter. Since the pattern of \u03b23 expression in DRG appears to correlate with the TTX\u2010resistant voltage\u2010gated sodium channel subunit PN3, we co\u2010expressed the two subunits in Xenopus oocytes. In this system, \u03b23 caused a 5\u2010mV hyperpolarizing shift in the threshold of activation of PN3, and a threefold increase in the peak current amplitude when compared with PN3 expressed alone. On the basis of these results, we examined the expression of \u03b2\u2010subunits in the chronic constriction injury model of neuropathic pain. Results revealed a significant increase in \u03b23 mRNA expression in small\u2010diameter sensory neurons of the ipsilateral DRG. These results show that \u03b23 is the dominant auxiliary sodium channel subunit in small\u2010diameter neurons of the rat DRG and that it is significantly upregulated in a model of neuropathic pain.<\/jats:p>","DOI":"10.1046\/j.1460-9568.2000.00294.x","type":"journal-article","created":{"date-parts":[[2003,3,6]],"date-time":"2003-03-06T14:28:18Z","timestamp":1046960898000},"page":"3985-3990","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":82,"title":["\u03b23, a novel auxiliary subunit for the voltage\u2010gated sodium channel, is expressed preferentially in sensory neurons and is upregulated in the chronic constriction injury model of neuropathic pain"],"prefix":"10.1111","volume":"12","author":[{"given":"B. S.","family":"Shah","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E. 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