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In the present study we evaluated the potential survival\u2010promoting effect of adenovirally administered GDNF on axotomized retinal ganglion cells. A single intravitreal injection [7\u2003\u00d7\u200310<jats:sup>7<\/jats:sup>\u2003plaque\u2010forming units (pfu) or 7\u2003\u00d7\u200310<jats:sup>8<\/jats:sup>\u2003pfu] of an adenoviral vector expressing the rat GDNF gene from a cytomegalovirus promoter enhanced retinal ganglion cell survival 14\u2003days after axotomy by 67 and 125%, respectively, when compared to control animals. Intraocular administration of the vector rescued 12.6 and 23%, respectively, of the retinal ganglion cells which would otherwise have died after axotomy. An increase in retinal GDNF protein and specific virally transduced GDNF mRNA expression was detected following intraocular vector application. Our data support previous findings showing that adenoviral delivery of neurotrophic factors to the vitreous body is a feasible approach for the prevention of axotomy\u2010induced retinal ganglion cell death <jats:italic>in vivo<\/jats:italic> and may constitute a relevant strategy for future treatment in traumatic brain injury and ensuing neurodegeneration.<\/jats:p>","DOI":"10.1046\/j.1460-9568.2002.01893.x","type":"journal-article","created":{"date-parts":[[2003,3,6]],"date-time":"2003-03-06T14:45:10Z","timestamp":1046961910000},"page":"637-643","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":62,"title":["Dose\u2010dependent rescue of axotomized rat retinal ganglion cells by adenovirus\u2010mediated expression of glial cell\u2010line derived neurotrophic factor<i>in vivo<\/i>"],"prefix":"10.1111","volume":"15","author":[{"given":"Christian","family":"Schmeer","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guido","family":"Straten","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sebastian","family":"K\u00fcgler","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Claude","family":"Gravel","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mathias","family":"B\u00e4hr","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stefan","family":"Isenmann","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2002,3,7]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1097\/00001756-199110000-00007"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/373339a0"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.14-07-04368.1994"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1089\/hum.1997.8.4-423"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj3260001"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1097\/00001756-199410000-00010"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.7.3978"},{"key":"e_1_2_6_9_1","doi-asserted-by":"crossref","first-page":"9126","DOI":"10.1523\/JNEUROSCI.20-24-09126.2000","article-title":"Protection by synergistic effects of adenovirus\u2010mediated X\u2010chromosome\u2010linked inhibitor of apoptosis and glial cell line\u2010derived neurotrophic factor gene transfer in the 1\u2010methyl\u20104\u2010phenyl\u20101,2,3,6\u2010tetrahydropyridine model of Parkinson's disease","volume":"24","author":"Eberhardt O.","year":"2000","journal-title":"J. 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