{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T18:56:50Z","timestamp":1791140210838,"version":"4.1.0"},"reference-count":34,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2003,1,8]],"date-time":"2003-01-08T00:00:00Z","timestamp":1041984000000},"content-version":"vor","delay-in-days":7,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Eur J of Neuroscience"],"published-print":{"date-parts":[[2003,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Previous studies have shown the existence of proliferating cells in explants from bovine (<jats:italic>Bos Taurus<\/jats:italic>) lateral ventricle walls that were maintained for several days <jats:italic>in vitro<\/jats:italic> in the absence of serum and growth factors. In this study we have characterized the nature of new cells and have assessed whether the insulin\u2010like growth factor\u20101 (IGF\u20101) receptor regulates their survival and\/or proliferation. The explants were composed of the ependymal layer and attached subependymal cells. Ependymal cells in culture were labelled with glial markers (S\u2010100, vimentin, GFAP, BLBP, 3A7 and 3CB2) and did not incorporate bromodeoxiuridine when this molecule was added to the culture media. Most subependymal cells were immunoreactive for \u03b2III\u2010tubulin, a neuronal marker, and did incorporate bromodeoxiuridine. Subependymal neurons displayed immunoreactivity for IGF\u20101 and its receptor and expressed IGF\u20101 mRNA, indicating that IGF\u20101 is produced in the explants and may act on new neurons. Addition to the culture media of an IGF\u20101 receptor antagonist, the peptide JB1, did not affect the incorporation of bromodeoxiuridine to proliferating subependymal cells. However, JB1 significantly increased the number of TUNEL positive cells in the subependymal zone, suggesting that IGF\u20101 receptor is involved in the survival of subependymal neurons. In conclusion, these findings indicate that neurogenesis is maintained in explants from the lateral cerebral ventricle of adult bovine brains and that IGF\u20101 is locally produced in the explants and may regulate the survival of the proliferating neurons.<\/jats:p>","DOI":"10.1046\/j.1460-9568.2003.02432.x","type":"journal-article","created":{"date-parts":[[2003,3,6]],"date-time":"2003-03-06T14:50:31Z","timestamp":1046962231000},"page":"205-211","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":28,"title":["Neurogenesis in explants from the walls of the lateral ventricle of adult bovine brain: role of endogenous IGF\u20101 as a survival factor"],"prefix":"10.1111","volume":"17","author":[{"given":"M.","family":"P\u00e9rez\u2010Mart\u00edn","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Cifuentes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. 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