{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,2]],"date-time":"2026-11-02T21:32:48Z","timestamp":1793655168911,"version":"4.5.1"},"reference-count":22,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2003,12,19]],"date-time":"2003-12-19T00:00:00Z","timestamp":1071792000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["enviromicro-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Environmental Microbiology"],"published-print":{"date-parts":[[2004,3]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Alcanivorax<\/jats:italic>\n                    is an alkane\u2010degrading marine bacterium which propagates and becomes predominant in crude\u2010oil\u2010containing seawater when nitrogen and phosphorus nutrients are supplemented. To identify the genes responsible for alkane degradation in this organism, two putative genes for alkane hydroxylases were cloned from\n                    <jats:italic>Alcanivorax borkumensis<\/jats:italic>\n                    SK2. They were named\n                    <jats:italic>alkB1<\/jats:italic>\n                    and\n                    <jats:italic>alkB2<\/jats:italic>\n                    . These genes were subsequently disrupted in\n                    <jats:italic>A. borkumensis<\/jats:italic>\n                    SK2, and the growth phenotypes of the disruptants were examined. The results indicate that the\n                    <jats:italic>alkB1<\/jats:italic>\n                    gene is responsible for the degradation of short\u2010chain\n                    <jats:italic>n<\/jats:italic>\n                    \u2010alkanes. A double mutant defective in both\n                    <jats:italic>alkB1<\/jats:italic>\n                    and\n                    <jats:italic>alkB2<\/jats:italic>\n                    was still able to grow on medium\u2010chain\n                    <jats:italic>n<\/jats:italic>\n                    \u2010alkanes, indicating that genes other than\n                    <jats:italic>alkB1<\/jats:italic>\n                    and\n                    <jats:italic>alkB2<\/jats:italic>\n                    are also involved in\n                    <jats:italic>n<\/jats:italic>\n                    \u2010alkane hydroxylation by\n                    <jats:italic>A. borkumensis<\/jats:italic>\n                    SK2.\n                  <\/jats:p>","DOI":"10.1046\/j.1462-2920.2003.00550.x","type":"journal-article","created":{"date-parts":[[2004,12,22]],"date-time":"2004-12-22T16:30:54Z","timestamp":1103733054000},"page":"191-197","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":75,"title":["Cloning and functional analysis of\n                    <i>alkB<\/i>\n                    genes in\n                    <i>Alcanivorax borkumensis<\/i>\n                    SK2"],"prefix":"10.1111","volume":"6","author":[{"given":"Akihiro","family":"Hara","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sang\u2010ho","family":"Baik","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kazuaki","family":"Syutsubo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Norihiko","family":"Misawa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Theo H. 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