{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,12]],"date-time":"2026-05-12T00:18:05Z","timestamp":1778545085733,"version":"3.51.4"},"reference-count":14,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2003,8,29]],"date-time":"2003-08-29T00:00:00Z","timestamp":1062115200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Ophthalmology"],"published-print":{"date-parts":[[2003,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:bold>Purpose\u2002<\/jats:bold> To date, our knowledge of the canine trabecular meshwork (TM) with regard to contractility is incomplete. It is important to understand the potential contractile capability within the TM and possible changes associated with spontaneous hypertensive glaucoma. To that end we have examined the presence of actin, including smooth muscle (SM) actin, in the normal and glaucomatous canine iridocorneal angle (ICA) morphologically and immunohistochemically.<\/jats:p><jats:p><jats:bold>Methods\u2002<\/jats:bold> Sections from the ICAs of 12 Beagles with inherited glaucoma (3\u00a0months to 6\u00a0years old) and age\u2010matched normal Beagles were treated with target retrieval, protein and power blocked and sequentially incubated with the primary antibody (rat anticanine SM actin) and the secondary antibody (rabbit antirat immunoglobulin), followed by peroxidase labeled streptavidin and incubation with substrate\u2010chromogen solution (AEC). Smooth muscle fibers that lined an artery within canine heart tissue were used as positive controls. Separate specimens were prepared for ultrastructual observation.<\/jats:p><jats:p><jats:bold>Results\u2002<\/jats:bold> Ultrastructurally, cells within the inner, posterior region of the corneoscleral TM and outer, posterior region of the uveal TM contained many microfilaments, 6\u00a0nm in diameter (i.e. actin). Immunohistochemistry demonstrated that cells within these regions possessed SM actin, having been greatest posteriorly, but extended anteriorly to a lesser extent. In the preglaucomatous affected dog the localization pattern for SM actin was identical to that seen in the normal dogs. With the progression of the disease the pattern disappeared.<\/jats:p><jats:p><jats:bold>Conclusions\u2002<\/jats:bold> The interior presence of myofibroblastic cells within the canine ICA suggests that these cells and the smooth muscle cells of the ciliary body along the same plane of orientation function to facilitate the removal of aqueous humor and are likely to be influenced by vascular mediators. The contractile apparatus for the ICA in the dog with inherited glaucoma appeared identical to that of the normal dog prior to expression of the disease, but weakened as the disease progressed.<\/jats:p>","DOI":"10.1046\/j.1463-5224.2003.00295.x","type":"journal-article","created":{"date-parts":[[2003,8,29]],"date-time":"2003-08-29T13:13:31Z","timestamp":1062162811000},"page":"205-209","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Localization of smooth muscle actin in the iridocorneal angle of normal and spontaneous glaucomatous beagle dogs"],"prefix":"10.1111","volume":"6","author":[{"given":"T. R.","family":"Ryland","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P. A.","family":"Lewis","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Chisholm","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K. N.","family":"Gelatt","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D. A.","family":"Samuelson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2003,8,29]]},"reference":[{"key":"e_1_2_5_2_2","first-page":"347","article-title":"Localization of smooth muscle myosin\u2010containing cells in the aqueous outflow pathway","volume":"31","author":"De Kater AW","year":"1990","journal-title":"Investigative Ophthalmology and Visual Science"},{"key":"e_1_2_5_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4835(91)90020-F"},{"key":"e_1_2_5_4_2","first-page":"1135","article-title":"Clinical manifestations of inherited glaucoma in the beagle","volume":"16","author":"Gelatt KN","year":"1977","journal-title":"Investigative Ophthalmology"},{"key":"e_1_2_5_5_2","first-page":"550","article-title":"Ultrastructural changes in the aqueous outflow apparatus of beagles with inherited glaucoma","volume":"30","author":"Samuelson DA","year":"1989","journal-title":"Investigative Ophthalmology and Visual Science"},{"key":"e_1_2_5_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1350-9462(99)00015-4"},{"key":"e_1_2_5_7_2","doi-asserted-by":"publisher","DOI":"10.1159\/000027471"},{"key":"e_1_2_5_8_2","first-page":"2515","article-title":"A relaxation of trabecular meshwork and ciliary muscle by release of nitric oxide","volume":"35","author":"Wiederholt M","year":"1994","journal-title":"Investigative Ophthalmology and Visual Science"},{"key":"e_1_2_5_9_2","first-page":"1765","article-title":"Identification of an extensive system of nitric oxide\u2010producing cells in the ciliary muscle and outflow pathways of the human eye","volume":"36","author":"Nathanson JA","year":"1995","journal-title":"Investigative Ophthalmology and Visual Science"},{"key":"e_1_2_5_10_2","doi-asserted-by":"publisher","DOI":"10.1076\/ceyr.18.5.375.5355"},{"key":"e_1_2_5_11_2","first-page":"168","volume-title":"Nitric Oxide and Endothelin in the Pathogenesis of Glaucoma.","author":"Wiederholt M","year":"1998"},{"key":"e_1_2_5_12_2","first-page":"178","volume-title":"Nitric Oxide and Endothelin in the Pathogenesis of Glaucoma.","author":"Ellis D","year":"1998"},{"key":"e_1_2_5_13_2","first-page":"966","article-title":"Inhibition of Na+\u2010K+\u2010ATPase by endothelin\u20101 in human non\u2010pigmented ciliary epithelial cells","volume":"296","author":"Prasanna G","year":"2001","journal-title":"Journal of Pharmacology and Experimental Therapeutics"},{"key":"e_1_2_5_14_2","doi-asserted-by":"publisher","DOI":"10.1089\/jop.1996.12.123"},{"key":"e_1_2_5_15_2","doi-asserted-by":"publisher","DOI":"10.1076\/ceyr.17.1.31.5256"}],"container-title":["Veterinary Ophthalmology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1046%2Fj.1463-5224.2003.00295.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1046\/j.1463-5224.2003.00295.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,13]],"date-time":"2023-10-13T04:43:17Z","timestamp":1697172197000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1046\/j.1463-5224.2003.00295.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2003,8,29]]},"references-count":14,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2003,9]]}},"alternative-id":["10.1046\/j.1463-5224.2003.00295.x"],"URL":"https:\/\/doi.org\/10.1046\/j.1463-5224.2003.00295.x","archive":["Portico"],"relation":{},"ISSN":["1463-5216","1463-5224"],"issn-type":[{"value":"1463-5216","type":"print"},{"value":"1463-5224","type":"electronic"}],"subject":[],"published":{"date-parts":[[2003,8,29]]},"assertion":[{"value":"2003-08-29","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}