{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T07:26:44Z","timestamp":1792740404450,"version":"4.5.0"},"reference-count":34,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2002,1,4]],"date-time":"2002-01-04T00:00:00Z","timestamp":1010102400000},"content-version":"vor","delay-in-days":460,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Neurochemistry"],"published-print":{"date-parts":[[2000,10]]},"abstract":"<jats:p> <jats:bold>Abstract:<\/jats:bold> Traditional microdialysis techniques provide qualitative  \ndata, although quantitative data are often required for pharmacodynamic  \nanalyses. This study evaluated a potentially useful<jats:italic>in vivo<\/jats:italic> delivery technique  \nto calibrate microdialysis probes for ethanol. We measured<jats:italic>in vivo<\/jats:italic> delivery  \nextraction fractions within subjects across 2 days and found no change over  \ntime. We tested the effect of diffusion direction on extraction fraction and  \nfound that it was higher for ethanol diffusion out of the probe than for  \ndiffusion into the probe, both<jats:italic>in vitro<\/jats:italic> and<jats:italic>in vivo<\/jats:italic>. The<jats:italic>in vivo<\/jats:italic> extraction  \nfraction ratio of diffusion<jats:sub>IN<\/jats:sub> versus diffusion<jats:sub>OUT<\/jats:sub> was  \n0.65 \u00b1 0.03. Finally, we predicted extracellular brain ethanol  \nconcentrations after 1 g\/kg ethanol administration using<jats:italic>in vivo<\/jats:italic> delivery,  \n\u201cno net flux\u201d dialysis, or<jats:italic>in vivo<\/jats:italic> delivery corrected for  \ndiffusion direction with the<jats:italic>in vivo<\/jats:italic> extraction fraction ratio. Both<jats:italic>in vivo<\/jats:italic>  \ndelivery and \u201cno net flux\u201d dialysis predicted brain concentrations  \nthat were approximately one\u2010third lower than blood concentrations, whereas the  \ncorrected<jats:italic>in vivo<\/jats:italic> delivery predicted extracellular concentrations very similar to blood concentrations. We conclude that microdialysis calibration methods for ethanol require a measure of extraction fraction for diffusion into the probe. Further studies are needed to establish whether this effect is common to other alcohols.<\/jats:p>","DOI":"10.1046\/j.1471-4159.2000.0751685.x","type":"journal-article","created":{"date-parts":[[2003,3,12]],"date-time":"2003-03-12T06:48:15Z","timestamp":1047451695000},"page":"1685-1693","source":"Crossref","is-referenced-by-count":32,"title":["Quantification of Ethanol Concentrations in the Extracellular Fluid of the Rat Brain"],"prefix":"10.1111","volume":"75","author":[{"given":"Donita L.","family":"Robinson","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jennifer A.","family":"Lara","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lane J.","family":"Brunner","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rueben A.","family":"Gonzales","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2002,1,4]]},"reference":[{"key":"e_1_2_5_3_2","first-page":"157","article-title":"Intracerebral microdialysis.","volume":"22","author":"Amberg G.","year":"1989","journal-title":"J. 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