{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,16]],"date-time":"2026-07-16T23:12:45Z","timestamp":1784243565709,"version":"3.55.0"},"reference-count":14,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2001,12,20]],"date-time":"2001-12-20T00:00:00Z","timestamp":1008806400000},"content-version":"vor","delay-in-days":233,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Neurochemistry"],"published-print":{"date-parts":[[2001,5]]},"abstract":"<jats:p>\u03b1\u2010Synuclein is a major component of Lewy bodies, a neuropathological feature of Parkinson's disease. Two \u03b1\u2010synuclein mutations, Ala53Thr and Ala30Pro, are associated with early onset, familial forms of the disease. Recently, synphilin\u20101, a protein found to interact with \u03b1\u2010synuclein by yeast two hybrid techniques, was detected in Lewy bodies. In this study we report the interaction of \u03b1\u2010synuclein and synphilin\u20101 in human neuroglioma cells using a sensitive fluorescence resonance energy transfer technique. We demonstrate that the C\u2010terminus of \u03b1\u2010synuclein is closely associated with the C\u2010terminus of\u00a0synphilin\u20101. A weak interaction occurs between the N\u2010terminus of \u03b1\u2010synuclein and synphilin\u20101. The familial Parkinson's disease associated mutations of \u03b1\u2010synuclein (Ala53Thr and Ala30Pro) also demonstrate a strong interaction between their C\u2010terminal regions and synphilin\u20101. However, compared with wild\u2010type \u03b1\u2010synuclein, significantly less energy transfer occurs between the C\u2010terminus of Ala53Thr \u03b1\u2010synuclein and synphilin\u20101, suggesting that the Ala53Thr mutation alters the conformation of \u03b1\u2010synuclein in relation to synphilin\u20101.<\/jats:p>","DOI":"10.1046\/j.1471-4159.2001.00301.x","type":"journal-article","created":{"date-parts":[[2003,3,12]],"date-time":"2003-03-12T06:53:35Z","timestamp":1047452015000},"page":"929-934","source":"Crossref","is-referenced-by-count":61,"title":["Interaction of \u03b1\u2010synuclein and synphilin\u20101: effect of Parkinson's disease\u2010associated mutations"],"prefix":"10.1111","volume":"77","author":[{"given":"Hibiki","family":"Kawamata","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pamela J.","family":"McLean","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nutan","family":"Sharma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bradley T.","family":"Hyman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2001,12,20]]},"reference":[{"key":"e_1_2_7_2_1","first-page":"879","article-title":"Aggregation of \u03b1\u2010synuclein in Lewy bodies of sporadic Parkinson's disease and dementia with Lewy bodies","volume":"152","author":"Baba M.","year":"1998","journal-title":"Am. 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