{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,13]],"date-time":"2026-02-13T08:20:57Z","timestamp":1770970857465,"version":"3.50.1"},"reference-count":35,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2001,12,20]],"date-time":"2001-12-20T00:00:00Z","timestamp":1008806400000},"content-version":"vor","delay-in-days":141,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Neurochemistry"],"published-print":{"date-parts":[[2001,8]]},"abstract":"<jats:p>Immunoelectron microscopy analysis of brain tissue sections and rat\u2010specific sandwich ELISA allowed the localization of interleukin\u20101\u03b2 (IL\u20101\u03b2) immunoreactivity in the mitochondria and cytosol of neocortical tissue preparations from the brain of naive, untreated, rats and rats receiving a single daily injection into one lateral cerebral ventricle (i.c.v.) of bovine serum albumin (BSA; 100\u2003ng\/day) for seven consecutive days. Interestingly, seven days i.c.v. treatment with the HIV\u20101 coat protein gp120 (100\u2003ng\/day) enhances IL\u20101\u03b2 immunoreactivity in the cellular fractions studied. Elevation of mitochondrial immunoreactive IL\u20101\u03b2 levels seems to originate from the conversion operated by the interleukin converting enzyme (ICE) of mitochondrial pro\u2010IL\u20101\u03b2; in fact, IL\u20101\u03b2 increases reported in the ELISA experiments were paralleled by a decrease of the mitochondrial pro\u2010IL\u20101\u03b2 31\u2010kDa band in conjunction with enhanced expression of the p20 component of activated ICE. In conclusion, the present results demonstrate that gp120\u2010enhanced neocortical expression of IL\u20101\u03b2 originates, at least in part, from <jats:italic>in situ<\/jats:italic> cleavage of mitochondrial pro\u2010IL\u20101\u03b2 and suggest that this, together with the central role of the mitochondrion in the expression of programmed cell death, may be important for apoptosis induced by the viral coat protein in the brain of rats.<\/jats:p>","DOI":"10.1046\/j.1471-4159.2001.00441.x","type":"journal-article","created":{"date-parts":[[2003,3,12]],"date-time":"2003-03-12T06:55:26Z","timestamp":1047452126000},"page":"611-618","source":"Crossref","is-referenced-by-count":22,"title":["Evidence that increases of mitochondrial immunoreactive IL\u20101\u03b2 by HIV\u20101 gp120 implicate <i>in situ<\/i> cleavage of pro\u2010IL\u20101\u03b2 in the neocortex of rat"],"prefix":"10.1111","volume":"78","author":[{"given":"M. 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