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The extent of the release by 0.1\u2003\u00b5<jats:sc>m<\/jats:sc>ionomycin was in a range similar to that by 30\u2003m<jats:sc>m<\/jats:sc>KCl. The ionomycin (0.1\u2003\u00b5<jats:sc>m<\/jats:sc>)\u2010evoked [<jats:sup>3<\/jats:sup>H]GABA release was dose\u2010dependently inhibited by NO synthase inhibitors and hemoglobin, indicating that the ionomycin\u2010evoked [<jats:sup>3<\/jats:sup>H]GABA release is mediated through NO formation. The inhibition of cGMP formation by 1<jats:italic>H<\/jats:italic>\u2010[1,2,4] oxodizao [4,3\u2010a] quinoxalin\u20101\u2010one (ODQ), a selective inhibitor for NO\u2010sensitive guanylate cyclase, showed no affects on the ionomycin\u2010evoked [<jats:sup>3<\/jats:sup>H]GABA release. Tetrodotoxin and dibucaine significantly suppressed the ionomycin\u2010evoked [<jats:sup>3<\/jats:sup>H]GABA release and ionomycin increased fluorescence intensity of bis\u2010oxonol, suggesting the involvement of membrane depolarization in this release. The ionomycin\u2010evoked [<jats:sup>3<\/jats:sup>H]GABA release was maximally reduced by about 50% by GABA uptake inhibitors. The concomitant presence of nifedipine and \u03c9\u2010agatoxin VIA (\u03c9\u2010ATX), inhibitors for L\u2010 and P\/Q\u2010type voltage\u2010dependent calcium channels, respectively, caused the reduction in the ionomycin\u2010evoked release by about 50%. The simultaneous addition of nifedipine, \u03c9\u2010ATX and nipecotic acid completely abolished the release. Although ionomycin released glutamate, (+)\u20105\u2010methyl\u20101\u2010,11\u2010dihydro\u20105H\u2010dibenzo\u2010[a,d]cycloheptan\u20105,10\u2010imine (MK\u2010801) and 6,7\u2010 dinitroquinoxaline\u20102,3\u2010dione (DNQX) showed no effects on the ionomycin\u2010induced [<jats:sup>3<\/jats:sup>H]GABA release. Based on these results, it is concluded that NO formed by ionomycin plays a critical role in ionomycin\u2010evoked [<jats:sup>3<\/jats:sup>H]GABA release from the neurons.<\/jats:p>","DOI":"10.1046\/j.1471-4159.2002.00810.x","type":"journal-article","created":{"date-parts":[[2003,3,12]],"date-time":"2003-03-12T06:59:42Z","timestamp":1047452382000},"page":"130-141","source":"Crossref","is-referenced-by-count":3,"title":["Functional significance of nitric oxide in ionomycin\u2010evoked [<sup>3<\/sup>H]GABA release from mouse cerebral cortical neurons"],"prefix":"10.1111","volume":"81","author":[{"given":"Masashi","family":"Katsura","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Keijiro","family":"Shuto","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yutaka","family":"Mohri","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Makoto","family":"Shigeto","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Seitaro","family":"Ohkuma","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2002,3,25]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/jnr.490380607"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1097\/00001756-199511000-00035"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0306-4522(96)00670-7"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-4695(199701)32:1<11::AID-NEU2>3.0.CO;2-H"},{"key":"e_1_2_7_6_1","doi-asserted-by":"crossref","first-page":"6229","DOI":"10.1016\/S0021-9258(18)50350-1","article-title":"The effect of ionomycin on calcium influxes in sarcoplasmic reticulum vesicles and liposomes","volume":"81","author":"Beeler T. 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