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In this study, the roles of endogenous Bcl\u20102 proteins in cyanide\u2010induced apoptosis were investigated. After cyanide (100\u2013500\u2003\u00b5<jats:sc>m<\/jats:sc>) treatment for 24\u2003h, two pro\u2010apoptotic Bcl\u20102 proteins, Bcl\u2010X<jats:sub>S<\/jats:sub>and Bax were up\u2010regulated as shown by western blot and RT\u2010PCR analysis. The expression levels of two antiapoptotic Bcl\u20102 proteins, Bcl\u20102 and Bcl\u2010X<jats:sub>L<\/jats:sub>, remained unchanged after cyanide treatment, whereas the mRNA levels of Bcl\u2010X<jats:sub>S<\/jats:sub>and Bax began to increase within 2\u2003h and their protein levels increased 6\u2003h after treatment. NF\u2010\u03baB, a redox\u2010sensitive transcription factor activated after cyanide treatment, is responsible for the up\u2010regulation of Bcl\u2010X<jats:sub>S<\/jats:sub>and Bax. SN50, which is a synthetic peptide that blocks translocation of NF\u2010\u03baB from cytosol to nucleus, inhibited the up\u2010regulation of Bcl\u2010X<jats:sub>S<\/jats:sub>and Bax. Similar results were obtained using a specific \u03baB decoy DNA. NMDA receptor activation and reactive oxygen species (ROS) generation are upstream events of NF\u2010\u03baB activation, as blockade of these two events by MK801,<jats:sc>l<\/jats:sc>\u2010NAME or PBN inhibited cyanide\u2010induced up\u2010regulation of Bcl\u2010X<jats:sub>S<\/jats:sub>and Bax. Up\u2010regulation of pro\u2010apoptotic Bcl\u2010X<jats:sub>S<\/jats:sub>and Bax contributed to cyanide\u2010induced cytochrome<jats:italic>c<\/jats:italic>release, because SN50 and a specific Bax antisense oligodeoxynucleotide significantly reduced release of cytochrome<jats:italic>c<\/jats:italic>from mitochondria as shown by western blot analysis. It was concluded that NF\u2010\u03baB\u2010mediated up\u2010regulation of Bcl\u2010X<jats:sub>S<\/jats:sub>and Bax is involved in regulating cytochrome<jats:italic>c<\/jats:italic>release in cyanide\u2010induced apoptosis.<\/jats:p>","DOI":"10.1046\/j.1471-4159.2002.00880.x","type":"journal-article","created":{"date-parts":[[2003,3,12]],"date-time":"2003-03-12T07:00:46Z","timestamp":1047452446000},"page":"842-852","source":"Crossref","is-referenced-by-count":95,"title":["NF\u2010\u03baB\u2010mediated up\u2010regulation of Bcl\u2010X<sub>S<\/sub>and Bax contributes to cytochrome<i>c<\/i>release in cyanide\u2010induced apoptosis"],"prefix":"10.1111","volume":"81","author":[{"given":"Yan","family":"Shou","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nianyu","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joseph L.","family":"Borowitz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gary E.","family":"Isom","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2002,5,7]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.281.5381.1322"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.92.20.9328"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(93)90508-N"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.20.8.2687-2695.2000"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.144.5.891"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-8993(97)01040-8"},{"key":"e_1_2_7_8_1","doi-asserted-by":"crossref","first-page":"4388","DOI":"10.4049\/jimmunol.153.10.4388","article-title":"Cloning and molecular characterization of mouse Bcl\u2010x in B and T lymphocytes","volume":"153","author":"Fang W.","year":"1994","journal-title":"J. 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