{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,30]],"date-time":"2026-10-30T23:13:56Z","timestamp":1793402036638,"version":"4.5.0"},"reference-count":71,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2002,7,24]],"date-time":"2002-07-24T00:00:00Z","timestamp":1027468800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Neurochemistry"],"published-print":{"date-parts":[[2002,8]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Comparison of [\n                    <jats:sup>125<\/jats:sup>\n                    I]epibatidine and 5\u2010[\n                    <jats:sup>125<\/jats:sup>\n                    I]iodo\u20103\u2010(2\u2010azetidinylmethoxy)pyridine ([\n                    <jats:sup>125<\/jats:sup>\n                    I]A\u201085380) autoradiography showed evidence for nicotinic receptor heterogeneity. To identify the receptor subtypes, we performed [\n                    <jats:sup>125<\/jats:sup>\n                    I]epibatidine autoradiography in the presence of cytisine or A\u201085380. By comparing these results with binding data from human embryonic kidney (HEK) 293 cells stably transfected with different combinations of rat nicotinic receptor subunits, we were able to quantify three distinct populations of [\n                    <jats:sup>125<\/jats:sup>\n                    I]epibatidine binding sites with characteristics of \u03b14\u03b22, \u03b13\u03b22 and \u03b13\u03b24 receptors. Although the predominant subtype in rat brain was \u03b14\u03b22, non\u2010\u03b14\u03b22 binding sites were prominent in many regions. In the habenulo\u2010peduncular system, cerebellum, substantia gelatinosa, and many medullary nuclei, \u03b13\u03b24\u2010like binding accounted for more than 40% of [\n                    <jats:sup>125<\/jats:sup>\n                    I]epibatidine binding, and nearly all binding in superior cervical ganglion and pineal gland. Other regions enriched in \u03b13\u03b24\u2010like binding included locus ceruleus, dorsal tegmentum, subiculum and anteroventral thalamic nucleus. Regions enriched in \u03b13\u03b22\u2010like binding included the habenulo\u2010peduncular system, many visual system structures, certain geniculate nuclei, and dopaminergic regions. The combination of autoradiography using a broad spectrum radioligand in the presence of selectivecompetitors, and data from binding to defined receptor subtypes in expression systems, allowed us to quantify the relative populations of these three subtypes.\n                  <\/jats:p>","DOI":"10.1046\/j.1471-4159.2002.00951.x","type":"journal-article","created":{"date-parts":[[2003,3,12]],"date-time":"2003-03-12T02:01:53Z","timestamp":1047434513000},"page":"468-481","source":"Crossref","is-referenced-by-count":222,"title":["Measuring nicotinic receptors with characteristics of \u03b14\u03b22, \u03b13\u03b22 and \u03b13\u03b24 subtypes in rat tissues by autoradiography"],"prefix":"10.1111","volume":"82","author":[{"given":"David C.","family":"Perry","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yingxian","family":"Xiao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Henry N.","family":"Nguyen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John L.","family":"Musachio","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martha I.","family":"D\u00e1vila\u2010Garc\u00eda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kenneth J.","family":"Kellar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2002,7,24]]},"reference":[{"key":"e_1_2_9_2_1","first-page":"563","article-title":"Epibatidine, a potent analgetic and nicotinic agonist","volume":"45","author":"Badio B.","year":"1994","journal-title":"Mol. 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