{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,18]],"date-time":"2023-10-18T06:36:04Z","timestamp":1697610964946},"reference-count":38,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2002,7,24]],"date-time":"2002-07-24T00:00:00Z","timestamp":1027468800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Neurochemistry"],"published-print":{"date-parts":[[2002,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>There are tissue specific discrepancies in expression of tryptophan hydroxylase (TPH) between the pineal gland and brainstem. TPH mRNA levels in the pineal are much higher than in the brainstem, however, the two tissues contain comparable protein levels. This discrepancy could result from different translation efficiency of two of the TPH mRNA isoforms. Using PCR\u2010based methods, we analyzed the relative expression, in pineal and brainstem, of two TPH mRNA isoforms differing in the length of their untranslated region (5\u2032UTR). The levels of the TPH\u03b1 were found to be 960\u2010fold more abundant than the 51\u2010nucleotide longer TPH\u03b2, in the pineal. TPH\u03b2 was also detected for the first time in the brainstem, where TPH\u03b2\/TPH\u03b1 was about five\u2010fold higher than in the pineal. To study the role of the different 5\u2032UTRs, each was cloned in\u2010frame upstream of luciferase, and transfected into PC12 cells. Both 5\u2032UTRs enhanced luciferase activity, with TPH\u03b2 5\u2032UTR being more effective than TPH\u03b1 5\u2032UTR, indicating selective regulation of translation efficiency. We also examined whether physiological manipulations alter the distribution of the TPH mRNA isoforms. Repeated stress had no effect in pineal, but led to a marked preferential induction of TPH\u03b2 in brainstem. Modulation of TPH gene expression in serotonergic neurons could result from selective and tissue specific regulation of its mRNA isoforms.<\/jats:p>","DOI":"10.1046\/j.1471-4159.2002.00989.x","type":"journal-article","created":{"date-parts":[[2003,3,12]],"date-time":"2003-03-12T07:01:53Z","timestamp":1047452513000},"page":"645-654","source":"Crossref","is-referenced-by-count":16,"title":["Role of the 5\u2032 untranslated region (UTR) in the tissue\u2010specific regulation of rat tryptophan hydroxylase gene expression by stress"],"prefix":"10.1111","volume":"82","author":[{"given":"Firas","family":"Chamas","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Esther L.","family":"Sabban","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2002,7,24]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"crossref","first-page":"5041","DOI":"10.1523\/JNEUROSCI.13-12-05041.1993","article-title":"Increase of tryptophan hydroxylase enzyme protein by dexamethasone in adrenalectomized rat midbrain","volume":"13","author":"Azmitia E. 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