{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,25]],"date-time":"2025-10-25T11:50:07Z","timestamp":1761393007349},"reference-count":35,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2002,7,24]],"date-time":"2002-07-24T00:00:00Z","timestamp":1027468800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Neurochemistry"],"published-print":{"date-parts":[[2002,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Synaptic\u2010like microvesicles (SLMVs) are morphological and functional equivalents of neuronal synaptic vesicles, and are responsible for the storage and secretion of classical neurotransmitters in various endocrine cells. Vacuolar H<jats:sup>+<\/jats:sup>\u2010ATPase acidifies the internal space of these organelles and provides a driving force for the uptake of neurotransmitters. Thus, the luminal pH is an important determinant of the function of SLMVs, although its value in living cells is unknown. Here, we determined the luminal pH of SLMVs in living rat pinealocytes by means of an immunoelectronmicroscopic procedure basedon the distribution of an amphipathic amine, 3\u2010(2,4\u2010dinitroanilino)\u20103\u2032\u2010amino\u2010<jats:italic>N<\/jats:italic>\u2010methyldipropylamine (DAMP). Use of double\u2010labeling techniques with antibodies against 2,4\u2010dinitrophenol for DAMP and synaptophysin for SLMVs, and of frozen ultrathin sections enabled us to determine the number of immunogold particles for DAMP per \u00b5m<jats:sup>2<\/jats:sup> of SLMVs. Using the density of gold particles, the luminal pH of SLMVs was calculated to be 5.11\u2003\u00b1\u20030.01. Treatment with either 1\u2003\u00b5<jats:sc>m<\/jats:sc> bafilomycin A1, a specific inhibitor of vacuolar H<jats:sup>+<\/jats:sup>\u2010ATPase , or 50\u2003m<jats:sc>m<\/jats:sc> ammonium chloride, a dissipater of the transmembrane pH gradient, increased the luminal pH to 6.04\u2003\u00b1\u20030.07 and 6.05\u2003\u00b1\u20030.11, respectively. Simultaneously, the lysosomal pH was found to be 5.14\u2003\u00b1\u20030.07, which increased to 5.77\u2003\u00b1\u20030.09 and 5.93\u2003\u00b1\u20030.13 with bafilomycin A1 and ammonium chloride, respectively. It is concluded that the luminal pH of SLMVs is comparable to that of lysosomes <jats:italic>in vivo<\/jats:italic>.<\/jats:p>","DOI":"10.1046\/j.1471-4159.2002.01025.x","type":"journal-article","created":{"date-parts":[[2003,3,12]],"date-time":"2003-03-12T07:01:53Z","timestamp":1047452513000},"page":"698-704","source":"Crossref","is-referenced-by-count":7,"title":["The internal pH of synaptic\u2010like microvesicles in rat pinealocytes in culture"],"prefix":"10.1111","volume":"82","author":[{"given":"Mitsuko","family":"Hayashi","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Akitsugu","family":"Yamamoto","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoshinori","family":"Moriyama","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2002,7,24]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1053\/gast.1996.v110.pm8613067"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.81.15.4838"},{"key":"e_1_2_7_4_1","first-page":"34","article-title":"Properties of acidified compartments in hippocampal neurons","volume":"61","author":"Augenbraun E.","year":"1993","journal-title":"Eur. 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