{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,18]],"date-time":"2023-10-18T22:54:48Z","timestamp":1697669688057},"reference-count":11,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2002,8,5]],"date-time":"2002-08-05T00:00:00Z","timestamp":1028505600000},"content-version":"vor","delay-in-days":4,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Helicobacter"],"published-print":{"date-parts":[[2002,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:bold>Background.<\/jats:bold> In murine <jats:italic>Helicobacter<\/jats:italic> infection it has been demonstrated that the degree of gastric mucosal inflammation is determined by mouse strain. This is a valuable tool for investigating genetic, immunological or bacterial determinants for the outcome of human <jats:italic>Helicobacter<\/jats:italic> infection. This study aims to devise a robust method to facilitate these investigations.<\/jats:p><jats:p><jats:bold>Materials and Methods.<\/jats:bold> C57BL\/6, BALB\/c and (C57BL\/6\u00a0\u00d7\u00a0BALB\/c) F1 mice were given 10<jats:sup>8<\/jats:sup><jats:italic>H.\u00a0felis<\/jats:italic> by gavage on days 1, 3 and 5 of the experiment Sections of the lesser and greater curve of stomach were examined at 12\u00a0weeks to assess active gastritis using a semi\u2010quantitative and a quantitative method by counting neutrophils in glands in four zones of the mucosa.<\/jats:p><jats:p><jats:bold>Results.<\/jats:bold> Semi\u2010quantitative scoring for active inflammation, based on the Sydney System, was inadequate with little discrimination between strains. Quantifying the level of active inflammation, counting the number of neutrophils in inflamed gastric glands and taking the total number of neutrophils in three inflamed pits within each zone (cardia, body, transitional zone and antrum) showed clear differences between the two parental strains for the degree of active gastritis and furthermore, using this system the phenotype of the (C57BL\/6\u00a0\u00d7\u00a0BALB\/c) F1 was found to be between the two parental extremes.<\/jats:p><jats:p><jats:bold>Conclusions.<\/jats:bold> This novel method provides a numerical value for active inflammation in the stomach that is accurate, reproducible and discriminatory.<\/jats:p>","DOI":"10.1046\/j.1523-5378.2002.00091.x","type":"journal-article","created":{"date-parts":[[2003,3,11]],"date-time":"2003-03-11T23:23:25Z","timestamp":1047425005000},"page":"265-268","source":"Crossref","is-referenced-by-count":7,"title":["A Novel Method for Assessing Gastritis in the Murine Model Demonstrates Genetically Determined Variation in Response to <i>Helicobacter felis<\/i> Infection"],"prefix":"10.1111","volume":"7","author":[{"given":"M. M.","family":"Walker","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Worku","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Coggle","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. R.","family":"Thursz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2002,8,5]]},"reference":[{"key":"e_1_2_5_2_2","first-page":"1273","article-title":"Unidentified curved bacilli on gastric epithelium in active chronic gastritis","author":"Warren JR","year":"1983","journal-title":"Lancet"},{"key":"e_1_2_5_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(84)91816-6"},{"key":"e_1_2_5_4_2","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.302.6788.1302"},{"key":"e_1_2_5_5_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199405053301803"},{"key":"e_1_2_5_6_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-695X.1999.tb01290.x"},{"key":"e_1_2_5_7_2","doi-asserted-by":"publisher","DOI":"10.1136\/gut.39.5.639"},{"key":"e_1_2_5_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0016-5085(97)70155-0"},{"key":"e_1_2_5_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.64.1.238-245.1996"},{"key":"e_1_2_5_10_2","first-page":"217","article-title":"MALToma\u2010like lesions in the murine gastric mucosa after long\u2010term infection with Helicobacter felis. A mouse model of Helicobacter pylori\u2010induced gastric lymphoma","volume":"147","author":"Enno A","year":"1995","journal-title":"Am J Pathol"},{"key":"e_1_2_5_11_2","doi-asserted-by":"publisher","DOI":"10.1097\/00000478-199610000-00001"},{"key":"e_1_2_5_12_2","doi-asserted-by":"publisher","DOI":"10.1136\/gut.45.3.335"}],"container-title":["Helicobacter"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1046%2Fj.1523-5378.2002.00091.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1046\/j.1523-5378.2002.00091.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,17]],"date-time":"2023-10-17T16:55:37Z","timestamp":1697561737000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1046\/j.1523-5378.2002.00091.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2002,8]]},"references-count":11,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2002,8]]}},"alternative-id":["10.1046\/j.1523-5378.2002.00091.x"],"URL":"https:\/\/doi.org\/10.1046\/j.1523-5378.2002.00091.x","archive":["Portico"],"relation":{},"ISSN":["1083-4389","1523-5378"],"issn-type":[{"value":"1083-4389","type":"print"},{"value":"1523-5378","type":"electronic"}],"subject":[],"published":{"date-parts":[[2002,8]]}}}